Effects of Tea Tree Oil on Expression of Inflammatory Factors in Bovine Rumen Epithelial Cells Induced by Lipopolysaccharide
MA Xiaoyu1,2,3, PENG Cheng1,2,3, ZHAN Kang1,2,3, ZHAN Jinshun4, YANG Tianyu1,2,3, NING Lili1,2,3, ZHAO Guoqi1,2,3
1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China;
3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University Yangzhou 225009, China;
4. Institute of Animal Husbandry and Veterinary, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China
Abstract：The purpose of this study was to investigate the effects of tea tree oil (TTO) on the expression of inflammatory factors in bovine rumen epithelial cells (BRECs) induced by lipopolysaccharide (LPS). The cell counting kit-8 (CCK-8) was used to determine the effects of different TTO concentrations [0 (control), 0.006 25%, 0.012 5%, 0.025%, 0.05% and 0.1% TTO] on the activity of BRECs and each concentration was performed six replicates. The concentration of 0.05% TTO was selected according to the above tests for subsequent experiments. Then, qRT-PCR and Western blot were used to investigate the changes of expression levels of inflammatory factors at gene and protein levels in BRECs. The experiment was divided into four groups: control group (CON group, without adding TTO and LPS), TTO group (only adding 0.05% TTO), LPS group (only adding 1 μg/mL LPS), LPS+TTO group (both adding 1 μg/mL LPS and 0.05% TTO). Each group was performed six replicates. The results showed as follows: 1) compared with the CON group, the cell viability of BRECs was significantly improved by adding 0.05% TTO (P<0.05) and was significantly inhibited by adding 0.1% TTO (P<0.05). 2) Compared with the CON group, the expression levels of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α) and Toll like receptor-4 (TLR-4) genes were significantly increased when LPS was added (P<0.05). Compared with LPS group, the expression levels of IL-1β, IL-6, IL-8, TNF-α and TLR-4 genes were significantly decreased by adding TTO to LPS (P<0.05). 3) Compared with the CON group, LPS could increase the protein expression levels of p65 and p-p65 in nuclear factor-κB (NF-κB) pathway but adding both LPS and TTO could decrease the protein expression levels of p65 and p-p65. In summary, low concentration of TTO can enhance the activity of BRECs and inhibit the expression of proteins associated with inflammatory signaling pathway NF-κB induced by inflammation of BRECs which caused by LPS, and then can inhibit the production of proinflammatory factor.
马晓宇, 彭程, 詹康, 占今舜, 杨天宇, 宁丽丽, 赵国琦. 茶树油对脂多糖诱导的奶牛瘤胃上皮细胞炎症因子表达的影响[J]. 动物营养学报, 2020, 32(12): 5903-5909.
MA Xiaoyu, PENG Cheng, ZHAN Kang, ZHAN Jinshun, YANG Tianyu, NING Lili, ZHAO Guoqi. Effects of Tea Tree Oil on Expression of Inflammatory Factors in Bovine Rumen Epithelial Cells Induced by Lipopolysaccharide. Chinese Journal of Animal Nutrition, 2020, 32(12): 5903-5909.
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