Abstract：The aim of this study was to establish a UPLC-MS/MS method for rapid determination of five β-agonists in pork liver, beef and mutton. The five β-agonists in samples were extracted with 80% acetonitrile, purified by PRiME HLB solid phase extraction column, concentrated by nitrogen, and finally dissolved with 10% methanol. A gradient elution was performed using 10 mmol/L ammonium acetate aqueous solution-10 mmol/L ammonium acetate methanol (pH=5) as a mobile phase. The multi-reaction monitoring (MRM) mode was used for the determination, and internal standard method was used for quantification. The concentration of five β-agonists showed good linearity in the range of 0.5 to 50.0 ng/mL with the coefficient correlation (R2) between 0.999 63 to 0.999 96. At the standard addition levels of 0.5, 1.0 and 5.0 μg/kg, the average recovery ratio of five β-agonists was 93.7% to 106.1% in pork liver, 95.2% to 104.0% in beef, and 95.1% to 106.0% in mutton. The test indicated that the method showed good accuracy and repeatability. Based on S/N=3, the detection limit of five β-agonists was 0.01 to 0.04 μg/kg, and quantitation limit was 0.02 to 0.15 μg/kg. This method is simple, sensitive, accurate, and suitable for large quantities animal products detection analysis.
张苏珍, 王益军, 田蕴, 贺燕, 葛敏. 猪肝、牛肉、羊肉中5种β-受体激动剂残留的超高效液相色谱-串联质谱分析方法的建立[J]. 动物营养学报, 2018, 30(10): 4087-4094.
ZHANG Suzhen, WANG Yijun, TIAN Yun, HE Yan, GE Min. UPLC-MS/MS Method for the Determination of Five β-agonists Residue in Pork Liver, Beef and Mutton. Chinese Journal of Animal Nutrition, 2018, 30(10): 4087-4094.
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