Effects of Different Inositol Levels on β-Catenin and Hormone-Sensitive Triglayceride Lipase Expression in Skin of Rex Rabbits
LI Min1, CUI Yali1, WANG Binglei1, CHEN Baojiang2
1. College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071000, China;
2. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China
Abstract：The aim of the present study was to investigate the effects of different inositol levels in diets on the expression of β-catenin and hormone-sensitive triglayceride lipase (HSL) in skin of Rex rabbits. A total of 120 (40±1)-day-old Rex rabbits (half male and half female) were randomly divided into 4 groups with 30 rabbits per group. Rex rabbits in 4 groups were fed diets with different levels of inositol (0, 25, 50 and 75 mg/kg, respectively), and the experiment lasted for 3 months. The skin of abdomen, dorsocentral region, hip were collected on days 30 (2-month-old), 60 (3-month-old), 90 (4-month-old), respectively, and the protein expression and location of β-catenin and HSL were studied by immunohistochemistry and Western blot methods. The results showed that the β-catenin was widely expressed in hair follicle, and the hair root sheath cells and hair papilla all had brown positive reaction cells. HSL was showed a very obvious dark brown strong positive expression in hair root sheath cells, especially in inner root sheath cells. The addition of 50 mg/kg inositol could extremely significantly increase the mean gray value of β-catenin and HSL positive cells in the hair follicles of the dorsocentral region skin of 2- to 4-month-old Rex rabbits (P<0.01), extremely significantly increase the mean gray value of β-catenin and HSL positive cells in the hair follicles of the dorsocentral region, abdomen and hip skin of 4-month-old Rex rabbits (P<0.01). The results suggest that inositol can promote the growth of hair follicle by increasing the expresstion of β-catenin and HSL of 4-month-old Rex rabbits, and the effect is the best when dietary inositol level is 50 mg/kg in this experiment.
李敏, 崔亚利, 王丙雷, 陈宝江. 不同水平肌醇对獭兔皮肤中β-连环蛋白和激素敏感脂酶表达的影响[J]. 动物营养学报, 2017, 29(8): 2743-2750.
LI Min, CUI Yali, WANG Binglei, CHEN Baojiang. Effects of Different Inositol Levels on β-Catenin and Hormone-Sensitive Triglayceride Lipase Expression in Skin of Rex Rabbits. Chinese Journal of Animal Nutrition, 2017, 29(8): 2743-2750.
