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动物营养学报 2017, Vol. 29 Issue (9) :3184-3192    DOI: 10.3969/j.issn.1006-267x.2017.09.021
饲料营养 Feed science and technology 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
德氏乳杆菌对育肥猪血脂指标、胆固醇代谢和脂肪沉积相关酶活性及基因mRNA表达的影响
李瑞1,2, 侯改凤1,2, 韦良开1,2, 彭伟1,2, 潘杰1,2, 黄兴国1,2
1. 湖南农业大学动物科学技术学院, 长沙 410128;
2. 湖南畜禽安全生产协同创新中心, 长沙 410128
Effects of Lactobacillus delbrueckii on Serum Lipid Indexes, Enzyme Activities and Genes mRNA Expression Related to Cholesterol Metabolism and Fat Deposition of Finishing Pigs
LI Rui1,2, HOU Gaifeng1,2, WEI Liangkai1,2, PENG Wei1,2, PAN Jie1,2, HUANG Xingguo1,2
1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Co-Innovation Center of Animal Production Safety, Changsha 410128, China
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摘要 本试验旨在研究饲粮中添加德氏乳杆菌制剂对育肥猪血脂指标、肝脏胆固醇代谢和脂肪沉积相关酶活性、组织胆固醇代谢和脂肪沉积相关基因mRNA表达和皮下脂肪组织形态的影响。试验选用120头平均体重为(65.34±3.64)kg的健康"杜×(长×大)"育肥猪,随机分为2组,每组6个重复(栏),每个重复10头(公母各占1/2)。对照组饲喂基础饲粮,试验组饲喂在基础饲粮中添加0.10%德氏乳杆菌制剂的饲粮,预试期7 d,试验期42 d。结果显示:1)与对照组相比,试验组育肥猪的血清甘油三酯(TG)(P=0.08)和总胆固醇(TC)(P=0.06)含量均有降低趋势,血清总胆汁酸(TBA)含量显著降低(P<0.05)。2)与对照组相比,试验组育肥猪的肝脏羟甲基戊二酰辅酶A还原酶(HMGR)活性有降低趋势(P=0.07),胆固醇7α-羟化酶活性(CYP7A1)显著增加(P<0.05),脂肪甘油三酯脂酶(ATGL)活性极显著降低(P<0.01)。3)与对照组相比,试验组育肥猪的回肠胆汁酸结合蛋白(IBABP)的mRNA相对表达量有降低趋势(P=0.07),皮下脂肪过氧化物酶体增殖物激活受体γ(PPARγ)的mRNA相对表达量显著增加(P<0.05);皮下脂肪细胞直径有降低趋势(P=0.09)。结果提示,德氏乳杆菌可通过干扰育肥猪回肠对胆汁酸的吸收来调控肝脏胆固醇和脂肪代谢。
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关键词德氏乳杆菌   育肥猪   酶活性   基因表达     
Abstract: The experiment was conducted to study the effects of dietary Lactobacillus delbrueckii preparation on serum lipid indexes, enzyme activities in liver and genes mRNA expression in tissues related to cholesterol metabolism and fat deposition and histomorphology of subcutaneous fat of finishing pigs. One hundred and twenty healthy Duroc×(Landrace×Yorkshire) finishing pigs with average body weight of (65.34±3.64) kg were randomly divided into two groups with six replicates (pens) per group and ten pigs per replicate (males and females in half). Pigs in the control group were fed a basal diet, while others in the experimental group were fed the basal diet supplemented with 0.10% Lactobacillus delbrueckii preparation. The pre-test lasted for 7 days and the experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the contents of triglyceride (TG) (P=0.08) and total cholesterol (TC) (P=0.06) in serum of finishing pigs in the experimental group had a decrease trend, and the content of total bile acid (TBA) in serum was significantly decreased (P<0.05). 2) Compared with the control group, the activity of hydroxymethylglutaryl CoA reductases (HMGR) in liver of finishing pigs in the experimental group had a decrease trend (P=0.07), the activity of cholesterol-7alpha-hydroxylase (CYP7A1) was significantly increased (P<0.05), and the activity of adipose triglyceride lipase (ATGL) was significantly decreased (P<0.01). 3) Compared with the control group, the mRNA relative expression of ileal bile acid binding protein (IBABP) of finishing pigs in the experimental group had a decrease trend (P=0.07), and the mRNA relative expression of peroxisome proliferators activated receptor γ (PPARγ) in subcutaneous fat was significantly increased (P<0.05). Subcutaneous fat cell diameter had a decrease trend compared with the control group (P=0.09). The results indicate that Lactobacillus delbrueckii can regulate cholesterol and lipid metabolism in liver via influencing bile acid absorption in ileum of finishing pigs.
KeywordsLactobacillus delbrueckii,   finishing pigs,   enzyme activities,   genes expression     
收稿日期: 2017-02-22;
基金资助:

国家科技计划课题(2013BAD21B04);国家自然科学基金项目(31372322);湖南省战略性新兴产业项目(2014GK1034)

通讯作者 黄兴国,教授,博士生导师,E-mail:huangxi8379@aliyun.com     Email: huangxi8379@aliyun.com
作者简介: 李瑞(1987-),男,湖北潜江人,博士研究生,从事饲料资源开发与利用研究。E-mail:lirui181000@163.com
引用本文:   
. 德氏乳杆菌对育肥猪血脂指标、胆固醇代谢和脂肪沉积相关酶活性及基因mRNA表达的影响[J]. 动物营养学报, 2017,V29(9): 3184-3192
. Effects of Lactobacillus delbrueckii on Serum Lipid Indexes, Enzyme Activities and Genes mRNA Expression Related to Cholesterol Metabolism and Fat Deposition of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2017,V29(9): 3184-3192.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2017.09.021     或     http://www.chinajan.com/CN/Y2017/V29/I9/3184
 
[1] LI C,NIE S P,DING Q,et al.Cholesterol-lowering effect of Lactobacillus plantarum NCU116 in a hyperlipidaemic rat model[J].Journal of Functional Foods,2014,8:340-347.
[2] GUILLEVIC M,KOUBA M,MOUROT J.Effect of a linseed diet or a sunflower diet on performances,fatty acid composition,lipogenic enzyme activities and stearoyl-CoA-desaturase activity in pig[J].Livestock Science,2009,124(1/2/3):288-294.
[3] 李瑞.德氏乳杆菌对育肥猪胆固醇代谢的影响研究[D].硕士学位论文.长沙:湖南农业大学,2014.
[4] 侯改凤.德氏乳杆菌对育肥猪生长性能、猪肉品质及脂肪沉积影响研究[D].硕士学位论文.长沙:湖南农业大学,2015.
[5] 杨公社.猪生产学[M].北京:中国农业出版社,2002:234-236.
[6] 李瑞,侯改凤,刘明,等.德氏乳杆菌对育肥猪生产性能、血脂指标及粪和组织中总胆固醇和总胆汁酸含量的影响[J].动物营养学报,2015,27(1):247-255.
[7] MEUNIER A,LALANNE F,NICOLLE C,et al.Blood-cholesterol-lowering strain of Lactobacillus delbrueckii:United States Patent 8652461[P].2014-02-18.
[8] 张董燕,季海峰,王四新,等.猪源唾液乳杆菌对生长猪生长性能、粪中微生物数量及血清指标的影响[J].动物营养学报,2014,26(3):725-731.
[9] 张剑峰.牛磺酸、低聚木糖和益生菌对蛋鸡生产及胆固醇沉积的影响[D].硕士学位论文.南京:南京农业大学,2011.
[10] 谢宁.两株乳酸杆菌对高脂饮食大鼠胆固醇影响及相关机制研究[D].博士学位论文.长沙:中南大学,2011.
[11] 廖端芳,唐朝克.胆固醇逆向转运基础与临床[M].北京:科学出版社,2009:19-20.
[12] GOLDBERG I J,ECJEL R H,ABUMRAD N A.Regulation of fatty acid uptake into tissues:lipoprotein lipase-and CD36-mediated pathways[J].Journal of Lipid Research,2008,50(Suppl.1):S86-S90.
[13] 张明明,宋光耀.高胆固醇血症低密度脂蛋白受体基因突变的研究进展[J].河北医科大学学报,2011,32(7):866-868.
[14] ABISAMBRA J F,FIORELI T,PADMANABHAN J,et al.LDLR expression and localization are altered in mouse and human cell culture models of alzheimer's Disease[J].PLoS One,2010,5(1):e8556.
[15] KRAUSE B R,AUERBACH B J.Reverse cholesterol transport and future pharmacological approaches to the treatment of atherosclerosis[J].Current Opinion in Investigational Drugs,2001,2(3):375-381.
[16] 张慧平,孙福成,王抒.高密度脂蛋白与动脉粥样硬化和冠心病[J].中国动脉硬化杂志,2004,12(6):733-736.
[17] SHNEIDER B L,DAWSON P A,CHRISTIE D M,et al.Cloning and molecular characterization of the ontogeny of a rat ileal sodium-dependent bile acid transporter[J].Journal of Clinical Investigation,1995,95(4):745-754.
[18] XU G,SHNEIDER B L,SHEFER S,et al.Ileal bile acid transport regulates bile acid pool,synthesis,and plasma cholesterol levels differently in cholesterol-fed rats and rabbits[J].Journal of Lipid Research,2000,41(2):298-304.
[19] DE RODAS B Z,GILLILAND S E,MAXWELL C V.Hypocholesterolemic action of Lactobacillus acidophilus ATCC 43121 and calcium in swine with hypercholesterolemia induced by diet[J].Journal of Dairy Science,1996,79(12):2121-2128.
[20] 宋冰,刘学政.大黄素对KKAy糖尿病小鼠脂肪组织PPAY-α及PPAY-γ表达影响的实验研究[J].北京中医药大学学报,2012,35(10):692-695.
[21] 段颖,郭翔宇,孙文,等.番石榴叶提取物调节小鼠脂肪组织TNF-α、PPAR-γ基因表达改善肝脏糖调节机制研究[J].中华中医药杂志,2014,29(5):1622-1625.
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