Abstract：The aims of the present study were to investigate the effects of Fusarium toxins in naturally contaminated diet on antioxidant capacity and distribution and expression of interleukin-1β (IL-1β) and interleukin-6 (IL-6) in spleen of weaned piglets. Forty healthy female weaned piglets (Duroc×Landrace×Yorkshire) aged at 35 days with an average body weight of (8.45±0.94) kg were randomly allocated into 2 groups with 20 piglets per group. Piglets in control group were fed a basal diet, and the others in Fusarium toxins group were fed Fusarium toxins[0.90 mg/kg zearalenone (ZEN); 1.43 mg/kg deoxynivalenol (DON); 5.85 mg/kg fumonisin (FUM)] contaminated experimental diet. The experiment lasted for 35 days after 7 days adaptation. The results showed as follows:1) compared with the control group, Fusarium toxins significantly decreased the activities of glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in serum and spleen of weaned piglets (P<0.05), whereas significantly increased the content of malondialdehyde (MDA) in serum and spleen (P<0.05). 2) Fusarium toxins significantly reduced the white pulp zone in spleen of weaned piglets, enlarged the red pulp zone and appeared round small cavity, while only a few lymphocytes were seen in the peripheral lymphatic sheath. 3) The positive cells of IL-1β and IL-6 in spleen of weaned piglets were mainly distributed in the edge of the white pulp, and concentrated area of positive cells were found near the blood sinus in spleen after exposure to Fusarium toxins. 4) Compared with the control group, Fusarium toxins significantly increased the relative expression of IL-1β and IL-6 mRNA in spleen of weaned piglets (P<0.05). It is suggested that dietary Fusarium toxins has significant effects on antioxidant capacity in serum and spleen, and decreases the immune function in spleen by changing distribution and expression of IL-1β and IL-6 in spleen of weaned piglets.
程群, 姜淑贞, 陈宁波, 黄丽波, 张桂国, 杨维仁. 镰刀菌毒素对断奶仔猪脾脏抗氧化能力和白细胞介素-1β、白细胞介素-6分布和表达的影响[J]. 动物营养学报, 2017, 29(9): 3267-3276.
CHENG Qun, JIANG Shuzhen, CHEN Ningbo, HUANG Libo, ZHANG Guiguo, YANG Weiren. Effects of Fusarium Toxins on Antioxidant Capacity and Distribution and Expression of Interleukin-1β and Interleukin-6 in Spleen of Weaned Piglets. Chinese Journal of Animal Nutrition, 2017, 29(9): 3267-3276.
