Effects of Chitosan Oligosaccharide on Growth Performance, Serum Immune and Antioxidant Indexes of Mice
ZHANG Nannan1, LI Lanlan1, ZHANG Xiaoyun1, WEN Zhengshun2, ZOU Xiaoting1
1. Key Laboratory of Animal Nutrition and Feed Science of Agriculture, Feed Science Institute, Zhejiang University, Hangzhou 310058, China;
2. College of Food and Medicine, Zhejiang Ocean University, Zhoushan 316022, China
Abstract：This experiment was carried out to investigate the effects of gavage administration of different doses of chitosan oligosaccharides (COS) on growth performance, serum immunoglobulins contents, cytokines levels and antioxidant enzyme activities of mice. Sixty 5-week-old specific pathogen-free (SPF) ICR male mice were selected and randomly divided into 6 groups, including 1 control group and 5 experimental groups with 10 mice each. The mice in 5 experimental groups were given gavage administration of COS (dissolved in normal saline) at levels of 0.2, 0.4, 0.6, 0.8 and 1.0 g/kg BW, and the mice in control group were given gavage administration of the same volume of normal saline. The experiment lasted for 6 weeks. The results showed as follows:1) gavage administration of different doses of COS had no significant influence on body weight of mice (P>0.05). 2) Thymus index was significantly increased by gavage administration of 0.6 g/kg BW COS, and it was increased by 32.87% compared with the control group (P<0.05). 3)Gavage administration of 0.6 g/kg BW COS significantly increased the contents of serum immunoglobulin A (IgA) and immunoglobulin (IgM), and they were increased by 12.14% and 20.69%, respectively, compared with the control group (P<0.05). 4) No significant differences were found in serum interleukin-6 (IL-6), interleukin-10 (IL-10), interleukin-1β (IL-1β), transforming growth factor-β (TGF-β) and tumour necrosis factor-α (TNF-α) by gavage administration of different doses of COS (P>0.05). 5)The activity of myeloperoxidase (MPO) was significantly increased by gavage administration of 0.6 g/kg BW COS, and it was increased by 27.61% compared with the control group (P<0.05). The activity of peroxidase (POD) was significantly increased by gavage administration of 0.8 g/kg BW COS, and it was increased by 45.08% compared with the control group (P<0.05). In summary, under the conditions of this experiment, COS has function of promoting the development of immune organs and regulating serum immune and antioxidant indexes of mice.
