Abstract:In the present study, mouse peritoneal macrophages (MPM) induced by lipopolysaccharide (LPS) were used as an inflammation model to investigate the anti-inflammatory effect of total flavonoids from Allium mongolicum Regel. The CCK-8 method was used to select the concentrations of total flavonoids that could stimulate cell viability of MPM. Based on the concentrations, the cells were treated in control group, LPS group (stress model, 1 μg/mL LPS), total flavonoids low dosage group (25 μg/mL total flavonoids+1 μg/mL LPS), medium dosage group (50 μg/mL total flavonoids+1 μg/mL LPS) and high dosage group (100 μg/mL total flavonoids+1 μg/mL LPS). The Griess method was applied to examine the nitric oxide (NO) content in cell supernatant; the contents of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 in cell supernatant were examined by the method of ELISA; the mRNA expression levels of TNF-α, IL-6, IL-1β, IL-10 and nitric oxide synthase (iNOS) of cells were tested by reverse transcription and polymerase chain reaction (RT-PCR). The results showed as follows:1) compared with the control, the cell proliferation ratio was significantly increased when the concentration range of total flavonoids was 25.0 to 200.0 μg/mL (P<0.05). 2) Compared with the control, the content of NO in cell supernatant was significantly increased in LPS group (P<0.05); compared with LPS group, different concentrations of total flavonoids could inhibit NO production (P<0.05). 3) Compared with the control, the contents of TNF-α,IL-6,IL-1β,IL-10 in cell supernatant and mRNA expression levels of TNF-α, IL-6, IL-1β, IL-10, iNOS in cells were significantly increased in LPS group (P<0.05); compared with LPS group, when cells were treated with total flavonoids at concoctions of 25, 50 and 100 μg/mL, except for IL-1β in low dosage group, the contents of TNF-α, IL-6 and IL-1β in cell supernatant were significantly decreased (P<0.05), and IL-10 content was significantly increased (P<0.05); except for iNOS in high dosage group, total flavonoids at concoctions of 25, 50 and 100 μg/mL significantly inhibited the mRNA expression of TNF-α,IL-6,IL-1β,iNOS in cells (P<0.05), and trend to improve IL-10 mRNA expression, but the difference was not significant (P>0.05). In conclusion, total flavonoids from Allium mongolicum Regel have a significant anti-inflammatory effect on MPM induced by LPS.
王特日格乐, 王翠芳, 丹妮, 萨茹丽, 杜红喜, 郭春利, 哈斯额尔敦, 曹琪娜, 敖长金. 沙葱总黄酮对脂多糖诱导的小鼠腹腔巨噬细胞炎症介质的影响[J]. 动物营养学报, 2018, 30(9): 3702-3709.
WANG Terigele, WANG Cuifang, DAN Ni, SA Ruli, DU Hongxi, GUO Chunli, Khas-Erdene, CAO Qina, AO Changjin. Effects of Total Flavonoids from Allium mongolicum Regel on Inflammatory Mediators induced by Lipopolysaccharide of Mouse Peritoneal Macrophages. Chinese Journal of Animal Nutrition, 2018, 30(9): 3702-3709.
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