Effects of Combination of Selenium Yeast and Polysavone on Egg Quality, Antioxidant Ability and Cholesterol Metabolism of Hens during Late Period of Laying
YANG Yu, SUN Yu, SUN Baosheng, LU Yingjie, LI Jianhui, ZHANG Junzhen
College of Animal Science and Technology, Shanxi Agricultural University, Taigu 030801, China
Abstract：This experiment was conducted to research the effects of combination of different levels of selenium yeast and polysavone on egg quality, antioxidant ability and cholesterol metabolism of hens during late period of laying. Seven hundred and fifty-six 69-week-old Hyline Brown hens were randomly allocated into 7 groups with 6 replicates of 18 hens for each replication. Hens in the control group were fed a basal diet, and those in experimental groups were fed the basal diet supplemented with 0.2 mg/kg selenium yeast+500 mg/kg polysavone, 0.2 mg/kg selenium yeast+1 000 mg/kg polysavone, 0.6 mg/kg selenium yeast+500 mg/kg polysavone, 0.6 mg/kg selenium yeast+1 000 mg/kg polysavone, 1.0 mg/kg selenium yeast+500 mg/kg polysavone and 1.0 mg/kg selenium yeast+1 000 mg/kg polysavone, respectively. The preliminary experiment was lasted for 2 weeks and the formal experiment lasted for 8 weeks. The results showed as follows:1) dietary selenium yeast and polysavone significantly increased the laying rate (P<0.05), significantly reduced the ratio of feed to egg (P<0.05), and tended to improve the eggshell thickness, albumen height and Haugh unit of laying hens. 2) Dietary selenium yeast and polysavone extremely significantly increased the activities of plasma glutathione peroxidase (GSH-Px) and total superoxide dismutase, GSH-Px activity in liver, mRNA expression levels of GSH-Px1 and thioredoxin reductase in liver of laying hens (P<0.01). Dietary selenium yeast and polysavone extremely significantly reduced the triglyceride content and the 3-hydroxyl-3-methyl-glutarate monoacyl coenzyme A reductase mRNA expression level in liver of laying hens (P<0.01), extremely significantly increased the mRNA expression levels of cholesterol 7α-hydroxylase and sterol regulatory element binding protein in liver of laying hens (P<0.01), and extremely significantly increased the selenium content in yolk (P<0.01). 3) The combination of 0.6 mg/kg selenium yeast +1 000 mg/kg polysavone could extremely significantly increase plasma total antioxidant capacity (P<0.01), extremely significantly reduce the plasma malondialdehyde content (P<0.01), and extremely significantly decrease the contents of cholesterol and triglyceride in yolk and cholesterol content in liver of laying hens (P<0.01). It can be concluded that the diet supplemented with selenium yeast and polysavone can improve the performance, egg quality, antioxidant ability and cholesterol metabolism of laying hens. The best supplemental level is 0.6 mg/kg selenium yeast +1 000 mg/kg polysavone.
杨玉, 孙煜, 孙宝盛, 卢营杰, 李建慧, 张俊珍. 酵母硒和苜草素联用对产蛋后期蛋鸡蛋品质、抗氧化能力和胆固醇代谢的影响[J]. 动物营养学报, 2018, 30(10): 4095-4104.
YANG Yu, SUN Yu, SUN Baosheng, LU Yingjie, LI Jianhui, ZHANG Junzhen. Effects of Combination of Selenium Yeast and Polysavone on Egg Quality, Antioxidant Ability and Cholesterol Metabolism of Hens during Late Period of Laying. Chinese Journal of Animal Nutrition, 2018, 30(10): 4095-4104.
YUAN D,ZHAN X A,WANG Y X.Effects of selenium sources and levels on reproductive performance and selenium retention in broiler breeder,egg,developing embryo,and 1-day-old chick[J].Biological Trace Element Research,2011,144(1/2/3):705-714.
ANUT C,ORAWAN C,PIYANETE C.Effect of sodium selenite and Zinc-L-selenomethionine on performance and selenium concentrations in eggs of laying hens[J].Asian-Australasian Journal of Animal Science,2008,21(7):1048-1052.
[7]
ATTIA Y A,ABDALAH A A,ZEWEIL H S,et al.Effect of inorganic or organic selenium supplementation on productive performance,egg quality and some physiological traits of dual-purpose breeding hens[J].Czech Journal of Animal Science,2010,55(11):505-519.
[8]
SHI L G,XUN W J,YUE W B,et al.Effect of sodium selenite,Se-yeast and nano-elemental selenium on growth performance,Se concentration and antioxidant status in growing male goats[J].Small Ruminant Research,2011,96(1):49-52.
CHEN G,WU J,LI C.Effect of different selenium sources on production performance and biochemical parameters of broilers[J].Journal of Animal Physiology and Animal Nutrition,2014,98(4):747-754,doi:10.1111/jpn.12136.
[13]
ZHOU L,SHI Y H,GUO R,et al.Digital gene-expression profiling analysis of the cholesterol-lowering effects of alfalfa saponin extract on laying hens[J].PLoS One,2014,9(6):e98578.
[16]
PAVLOVI? Z,MILETI? I,JOKI??,et al.The effect of dietary selenium source and level on hen production and egg selenium concentration[J].Biological Trace Element Research,2009,131(3):263-270.
BJARNADOTTIR O,ROMERO R,BENDAHL P Q,et al.Targeting HMG-CoA reductase with statins in a window-of-opportunity breast cancer trial[J].Breast Cancer Research and Treatment,2013,138(2):449-508.
[24]
YEGANEH B,WIECHEC E,ANDE S R,et al.Targeting the mevalonate cascade as a new therapeutic approach in heart disease,cancer and pulmonary disease[J].Pharmacology & Therapeutics,2014,143(3):87-110.
[26]
PAPPAS A C,KARADAS F,SURAI P F,et al.The selenium intake of the female chicken influences the selenium status of her progeny[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2005,142(4):465-474.
[2]
WANG Y X,ZHAN X A,YUAN D,et al.Influence of dietary selenomethionine supplementation on performance and selenium status of broiler breeders and their subsequent progeny[J].Biological Trace Element Research,2011,143(3):1497-1507.
[5]
PILARCZYK B,JANKOWIAK D,TOMZA-MARCINIAK A,et al.Selenium concentration and glutathione peroxidase (GSH-Px) activity in serum of cows at different stages of lactation[J].Biological Trace Element Research,2012,147(1/2/3):91-96.
[10]
JING C L,DONG X F,WANG Z M,et al.Comparative study of DL-selenomethionine vs. sodium selenite and seleno-yeast on antioxidant activity and selenium status in laying hens[J].Poultry Science,2015,94(5):965-975.
[12]
DENG W,DONG X F,TONG J M,et al. Effects of an aqueous alfalfa extract on production performance,egg quality and lipid metabolism of laying hens[J].Journal of Animal Physiology and Animal Nutrition,2012,96(1):85-94.
[20]
INVERNIZZI G,AGAZZI A,FERRONI M,et al.Effects of inclusion of selenium-enriched yeast in the diet of laying hens on performance,eggshell quality,and selenium tissue deposition[J].Italian Journal of Animal Science,2013,12(1):el.
[27]
DELEZIE E,ROVERS M,VAN DER AA A,et al.Comparing responses to different selenium sources and dosages in laying hens[J].Poultry Science,2014,93(12):3083-3090.
