Abstract：This experiment was aimed to investigate effects of fresh feed and mixed diets on gut microbiota diversity in European mink (Mustela iutreola). Twenty European minks were selected and divided into 2 groups with 10 minks in each group, and were fed fresh feed and mixed diet, respectively. After slaughter, intestinal contents were collected for extract genome of intestinal bacteria. The high-throughput 16S rRNA gene sequencing technology was used to analyze and compare systematics level of different sequences for investigating intestine microbial diversity. The results showed as follows:in the hindgut of minks in this 2 groups, bacteria were ranked 6 phylum, the relative abundant of Firmicutes was the highest in the mink intestine, the relative abundant of fresh feed group and mixed diet group were 84.08% and 83.50% (P=0.36), respectively, followed by Proteobacteria (9.37% and 7.22%) (P=0.36), Actinobacteria (3.85% and 5.72%) (P=0.28), Bacteria_Uncalssified (1.69% and 2.18%) (P=0.47), Cyanobacteria (0.66% and 0.85%) (P=0.07), Bacteroidetes (0.11% and 0.32%) (P=0.36), the proportion of this 6 phylum to all bacteria was more than 99%. Lactobacillus was the most relative abundant bacteria, followed by Weissella (21.7%) in the mixed diet group.Weissella showed the highest relative abundance (32.2%), followed by Lactobacillus (22.2%) in the fresh feed group, and the other bacteria had the similar relative abundance. Principal coordinates analysis (PCoA) and cluster analysis revealed that there were no significant differences in the microbial community between the 2 groups (P>0.05). However, the number of individuals with significant difference in mixed diet group was more than that in fresh feed group. In conclusion, intestinal bacteria composition of European mink was similar when fed the same nutrition level diet, but not feed composition. However, different bacterial relative abundances are different, and vary individually in mixed diet group. The analysis of results indicate that the diet is the one of main factors affecting intestinal flora difference of minks.
刘晗璐, 钟伟, 司华哲, 李志鹏, 李光玉. 鲜饲料与混合饲粮对咖啡貂(Mustela iutreola)肠道微生物多样性的影响[J]. 动物营养学报, 2019, 31(1): 226-235.
LIU Hanlu, ZHONG Wei, SI Huazhe, LI Zhipeng, LI Guangyu. Effects of Fresh Feed and Mixed Diets on Gut Microbiota Diversity in European Mink (Mustela iutreola). Chinese Journal of Animal Nutrition, 2019, 31(1): 226-235.
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