Abstract：The present experiment was conducted to evaluate the effects of single and combined exposure of deoxynivalenol (DON) and aflatoxin B1 (AFB1) on intestinal microflora diversity and species abundance in mice. Ninety-six Kunming mice (male, 18-day-old) were randomly divided into 4 groups with 24 mice in each group. Mice in control group were fed physiological saline by gavage. Mice in DON group were fed 500 μg/kg DON by gavage. Mice in AFB1 group were fed 200 μg/kg AFB1 by gavage. Mice in DON+AFB1 group were fed 500 μg/kg DON+200 μg/kg AFB1 by gavage. The trial was conducted for 45 d. On the days 0 (before the experiment) and 45 of the experiment, the fresh dejections in the rectum from each group (each group was randomly taken 3 samples) were randomly taken under aseptic conditions, and then the changes of the structure and composition of the microbial community were analyzed using Illumina HiSeq high-throughput sequencing technology. The results showed that compared with the control group, the abundances of Bacteroidetes and Bacteroidia in DON, AFB1 and DON+AFB1 groups was significantly reduced (P<0.01), and the abundance of Bacteroidia in DON+AFB1 group was significantly lower than that in DON and AFB1 groups (P<0.01). Compared with the control group, the abundance of Deferribacteres in DON, AFB1 and DON+AFB1 groups was significantly reduced (P<0.01), while the abundance of Clostridia in DON, AFB1 and DON+AFB1 groups was significantly increased (P<0.01). Moreover, the abundance of Spirochaetes in DON and DON+AFB1 groups was significantly higher than that in control group (P<0.01). In conclusion, single and combined exposure of DON and AFB1 all can alter the abundances of intestinal microflora, decrease the abundances of beneficial bacteria, and increase the abundances of harmful bacteria. DON and AFB1 exhibit a significant interaction with synergistic effects.
刘锦芳, 储小燕, 刘秦, 张娅菲, 朱雷, 张咪, 陈铃霞, 刘明达, 冯士彬, 李玉, 吴金节, 王希春. 脱氧雪腐镰刀菌烯醇与黄曲霉毒素B1单一及联合染毒对小鼠肠道菌群的影响[J]. 动物营养学报, 2019, 31(2): 900-911.
LIU Jinfang, CHU Xiaoyan, LIU Qin, ZHANG Yafei, ZHU Lei, ZHANG Mi, CHEN Lingxia, LIU Mingda, FENG Shibin, LI Yu, WU Jinjie, WANG Xichun. Effects of Single and Combined Exposure of Deoxynivalenol and Aflatoxin B1 on Intestinal Microflora in Mice. Chinese Journal of Animal Nutrition, 2019, 31(2): 900-911.
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