枯草芽孢杆菌铜对先天性缺铜仔鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响

doi:10.3969/j.issn.1006-267x.2019.03.044

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摘要本试验旨在研究枯草芽孢杆菌铜对先天性缺铜仔鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响。试验选择108只自然交配5 d的清洁级SD大鼠，随机分为6个组，每组18个重复，每个重复1只大鼠。Ⅰ~Ⅲ组为硫酸铜组，饲粮总铜含量分别为1.5、3.0、6.0 mg/kg；Ⅳ~Ⅵ为枯草芽孢杆菌铜组，饲粮总铜含量分别为1.5、3.0、6.0 mg/kg。试验期为35 d。结果表明：1）Ⅲ组体重显著高于Ⅰ组（P<0.05），Ⅵ组体重、体长显著高于Ⅳ组（P<0.05），其他各组体重、体长、存活率差异不显著（P>0.05）。2）各组之间心脏指数、肝脏指数、脾脏指数、肾脏指数、肺脏指数差异均不显著（P>0.05）。3）Ⅱ、Ⅲ组大脑中铜沉积量显著高于Ⅰ组（P<0.05），Ⅲ组肝脏中铜沉积量显著高于Ⅰ组（P<0.05）。Ⅵ组大脑中铜沉积量显著高于Ⅴ组（P<0.05），Ⅵ组肝脏中铜沉积量显著高于Ⅳ组（P<0.05）。Ⅳ组大脑中铜沉积量显著高于Ⅰ组（P<0.05）。4）Ⅲ组血清铜蓝蛋白（CP）和碱性磷酸酶（AKP）活性显著或极显著高于Ⅰ、Ⅱ组（P<0.05或P<0.01），Ⅴ、Ⅵ组血清CP活性显著高于Ⅳ组（P<0.05），Ⅳ、Ⅴ组血清CP活性分别显著高于Ⅰ、Ⅱ组（P<0.05）。Ⅲ组血清谷丙转氨酶（ALT）、谷草转氨酶（AST）活性极显著高于Ⅰ组（P<0.01），Ⅵ组血清ALT活性显著高于Ⅳ组（P<0.05），Ⅳ组血清ALT活性显著高于Ⅰ组（P<0.05）。5）Ⅲ组血清谷胱甘肽过氧化物酶（GSH-Px）活性显著高于Ⅰ、Ⅱ组（P<0.05），Ⅲ组血清铜锌超氧化物歧化酶（CuZn-SOD）活性显著高于Ⅰ组（P<0.05）；Ⅵ组血清CuZn-SOD活性显著高于Ⅳ组（P<0.05）。由此可见，适量枯草芽孢杆菌铜对先天性缺铜仔鼠生长发育和抗氧化能力具有快速修复作用，其作用效果优于硫酸铜。

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关键词 ：枯草芽孢杆菌铜, 硫酸铜, 妊娠哺乳期大鼠, 生长发育, 抗氧化能力

Abstract：This experiment was conducted to study the effects of Bacillus subtilis copper on growth and development, organ indexes, copper deposition and serum biochemical and antioxidant indexes of offspring rats with congenital copper deficiency. A total of 108 clean grade pregnant SD rats were selected and randomly divided into 6 groups with 18 replicates per group and 1 rat per replicate. Groups Ⅰ to Ⅲ were copper sulfate groups, and the dietary total copper contents were 1.5, 3.0 and 6.0 mg/kg, respectively; groups Ⅳ to Ⅵ were Bacillus subtilis copper groups, and the dietary total copper contents were 1.5, 3.0 and 6.0 mg/kg, respectively. The experiment lasted for 35 days. The results showed as follows:1) the body weight of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05), the body weight and body length of group Ⅵ were significantly higher than those of group Ⅳ (P<0.05), and there were no significant differences in body weight, body length and survival rate among other groups (P>0.05). 2) There were no significant differences in heart index, liver index, spleen index, kidney index and lung index among all groups (P>0.05). 3) The copper deposition in brain of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ (P<0.05), the copper deposition in liver of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05). The copper deposition in brain of group Ⅵ was significantly higher than that of group Ⅴ (P<0.05), the copper deposition in liver of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05). The copper deposition in brain of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 4) The serum ceruloplasmin (CP) and alkaline phosphatase (AKP) activities of group Ⅲ were significantly higher than those of groups Ⅰ and Ⅱ (P<0.05 or P<0.01), the serum CP activity of groups Ⅴ and Ⅵ was significantly higher than that of group Ⅳ (P<0.05), and the serum CP activity of groups Ⅳ and Ⅴ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), respectively. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities of group Ⅲ were significantly higher than those of group Ⅰ (P<0.01), the serum ALT activity of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05), and the serum ALT activity of group Ⅳ was significantly higher than that of group Ⅰ (P<0.05). 5) The serum glutathione peroxidase (GSH-Px) activity of group Ⅲ was significantly higher than that of groups Ⅰ and Ⅱ (P<0.05), the serum copper-zinc superoxide dismutase (CuZn-SOD) activity of group Ⅲ was significantly higher than that of group Ⅰ (P<0.05); the serum CuZn-SOD activity of group Ⅵ was significantly higher than that of group Ⅳ (P<0.05). In conclusion, the appropriate amount of Bacillus Subtilis copper has a rapid repair effect on the growth and development and antioxidant capacity of offspring rats with congenital copper deficiency, and its effect is better than copper sulfate.

Key words： Bacillus subtilis copper copper sulfate pregnancy and lactation rats growth and development antioxidant capacity

收稿日期: 2018-08-22

PACS:

S816.7

基金资助:

国家水禽产业技术体系专项基金（CARS-43-11）；国家重点研发计划"绿色水禽高效安全养殖技术应用与示范"（2018YFD0501501）

通讯作者: 王宝维,教授,硕士生导师,E-mail:wangbw@qau.edu.cn E-mail: wangbw@qau.edu.cn

作者简介: 刘国栋(1994-),男,山东安丘人,硕士研究生,研究方向为营养与保健。E-mail:1373657529@qq.com

引用本文:

刘国栋, 王宝维, 黄燕萍, 葛文华, 张名爱, 岳斌, 李凯玲, 孔敏. 枯草芽孢杆菌铜对先天性缺铜仔鼠生长发育、器官指数、铜沉积量、血清生化和抗氧化指标的影响[J]. 动物营养学报, 2019, 31(3): 1367-1375.
LIU Guodong, WANG Baowei, HUANG Yanping, GE Wenhua, ZHANG Ming, YUE Bin, LI Kailing, KONG Min. Effects of Bacillus subtilis Copper on Growth and Development, Organ Indexes, Copper Deposition and Serum Biochemical and Antioxidant Indexes of Offspring Rats with Congenital Copper Deficiency. Chinese Journal of Animal Nutrition, 2019, 31(3): 1367-1375.

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http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2019.03.044 或 http://www.chinajan.com/CN/Y2019/V31/I3/1367

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