Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets
LIU Jingbo1,2, CAO Shanchuan1, YANG Yong1, ZHANG Hongfu2
1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Abstract:The present study was conducted to determine the effects of meal frequency on growth performance, carcass composition and muscular protein synthesis-related genes expression in suckling piglets. Sixteen piglets of a crossbred genotype[Duroc×(Landrace×Large White)] were obtained from 4 litters in 4 successive replicates and grouped into 8 pairs according to sex and body weight. At 4 days of age, piglets within each pair were offered the same amount of feed allowance either in 6 (M6 group, n=8) or 12 (M12 group, n=8) meals per day during a 3-week interventional period. The results showed as follows:1) compared with M12 group, the final body weight and average daily body weight at week 2, week 3 and during the whole trial in M6 group significantly increased (P<0.05), and the feed/gain at week 2, week 3 and during the whole trial significantly decreased (P<0.05). 2) Compared with M12 group, the carcass lean percentage and weight of longissimus muscle and semitendinosus muscle in M6 group significantly increased (P<0.05). 3) Compared with M12 group, the mRNA expression of insulin-like growth factor 1 (IGF1R) in M6 group had an increasing trend (P=0.05); compared with M12 group, the mRNA expression of insulin-like growth factor 1 receptor (IGF1R), mammalian target of rapamcin (mTOR), eukaryotic cells translate initiation factor 4E (EIF4E) and ribosomal protein S6 (RPS6) in M6 group significantly increased (P<0.05), and the mRNA expression of Ⅱ type B activated receptor (ACVR2B) and type Ⅰ activated kinase receptor (ALK5) in M6 group significantly decreased (P<0.05); compared with M12 group, the mRNA expression of myostatin (MSTN) in M6 group had no significant change (P>0.05). Collectively, reduced meal frequency results in accelerated carcass lean deposition and muscle hypotrophy, which may be mediated by activated IGF1-IGF1R-mTOR pathway and down-regulated expression of MSTN receptors.
刘静波, 曹山川, 杨勇, 张宏福. 饲喂频率对哺乳仔猪生长性能和骨骼肌蛋白质合成的影响[J]. 动物营养学报, 2019, 31(7): 3049-3057.
LIU Jingbo, CAO Shanchuan, YANG Yong, ZHANG Hongfu. Effects of Meal Frequency on Growth Performance and Muscle Protein Synthesis in Suckling Piglets. Chinese Journal of Animal Nutrition, 2019, 31(7): 3049-3057.
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