Effects of Alanyl-Glutamine Dipeptide Supplementation on Growth Performance, Antioxidant Status, Immune Response and Stress Resistance of Juvenile Yellow Catfish (Pelteobagrus fulvidraco)
LI Xue1, ZHANG Muzi1, LI Ming1, ZHANG Qian1, WANG Rixin1, JIANG Haibo2,3
1. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2. College of Animal Sciences, Guizhou University, Guiyang 550025, China;
3. Key Laboratory for Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, Guizhou University, Guiyang 550025, China
Abstract：A 56-day feeding trial was conducted to determine the alanyl-glutamine dipeptide (AGD) supplementation on growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish (Pelteobagrus fulvidraco). Juvenile yellow catfish with an average body weight of (1.98±0.01) g were randomly assigned to 5 groups with 3 replicates per group and 30 juvenile fish per replicate. Juvenile fish in the 5 groups were fed 5 experimental diets supplemented with 0 (control) 0.25%, 0.50%, 0.75% and 1.00% AGD, and the measured AGD contents in the 5 experimental diets were 0.08%, 0.23%, 0.56%, 0.69% and 1.09%, respectively. The results showed that the weight gain, specific growth rate, serum total protein, albumin, globulin, complement 3, complement 4 contents and superoxide dismutase, catalase, glutathione, lysozyme, alkaline phosphatase, acid phosphatase activities were firstly increased and then increased with dietary AGD supplemental level increasing, and above indexes in 0.50% and 0.75%AGD groups were significantly higher than those in control group (P<0.05). Diet supplemented different levels of AGD could significantly increase the serum total antioxidant capacity (P<0.05), but significantly decrease the serum malondialdehyde content (P<0.05). After 96 h post-challenge on ammonia exposure, the cumulative mortality, brain ammonia and glutamate contents in control group were significantly higher than those in each AGD supplementation groups (P<0.05). In conclusion, suitable level of AGD supplemented into diets can increase the growth performance, antioxidant status, immune response and stress resistance of juvenile yellow catfish. The optimal dietary AGD supplemental level is estimated to be 0.56% in the diet of juvenile yellow catfish by quadratic regression analysis base on specific growth rate. By a comprehensive consideration of growth performance, antioxidant status, immune response and stress resistance, the supplemental level of 0.50% to 0.75% AGD is recommended in diets of juvenile yellow catfish.
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