1. Animal Science College of South China Agriculture University, Guangzhou 510642, China;
2. Shenzhen Kingsino Technology Co., Ltd., Shenzhen 518106, China;
3. Zhuhai Modern Agricultural Development Center, Zhuhai 519000, China
Abstract：This study was conducted to study the effects of rice extracting complex sugar on the growth performance and intestinal morphology and function of weaned pigs without attractant. A total of 160 healthy and similar ternary weaned piglets at (26±2) days of age were randomly divided into 5 groups, 4 replicates in each group, 8 pigs per replicate. Group A was fed a standard diet, group B was fed a basal diet (without sweeteners, flavors, glucose and sucrose), group C was fed the standard diet+3% rice extracting complex sugar, group D was fed the basal diet+3% rice extracting complex sugar, group E was fed the basal diet+5% rice extracting complex sugar. The results showed as follows:1) compared with group A, there was no significant difference in average daily gain (ADG) and average daily feed intake (ADFI) in group B (P>0.05), and ADG and ADFI in group C were significantly increased (P<0.05); compared with group B, the difference of ADG, ADFI and feed/gain (F/G) were not significantly different (P>0.05), the ADG and ADFI in group E were significantly increased (P<0.05), and F/G was significantly decreased (P<0.05). 2) Compared with group A, the apparent digestibility of crude protein (CP) in group B was significantly lower (P<0.05), and the apparent digestibility of CP in group C was higher (P=0.069); compared with group B, the apparent digestibility of nutrients in group D was not significantly different (P>0.05), and the apparent digestibility of dry matter (DM), CP and ether extract (EE) in group E were significantly increased (P<0.05). 3) Compared with group A, the crypt depth (CD) in group B was significantly increased (P<0.05), villus height/crypt depth (V/C) was decreased (P=0.073), and the villus height (VH) and V/C in group C were significantly increased (P<0.05); Compared with group B, VH and CD in group D was not significantly different (P>0.05), V/C increased (P=0.053), and V/C in group E increased significantly (P<0.05). 4) Compared with group A, the mRNA expression of occludin in group B was significantly increased (P<0.05), the mRNA expression of closed loop protein-1 (ZO-1) in group C was significantly decreased, and the mRNA expression of occludin was significantly increased (P<0.05); Compared with group B, the mRNA expression of ZO-1 and occludin in group D were significantly increased (P<0.05), the mRNA expression of ZO-1 in group E was increased (P=0.075), and the mRNA expression of occludin was significantly decreased (P<0.05). 5) Compared with group A, serum total antioxidant capacity (T-AOC) was significantly lower than that in group B (P<0.05), serum T-AOC in group C was not significantly different (P>0.05); compared with group B, serum T-AOC in group D and group E were significantly increased (P<0.05). 6) There was no significant difference in ileal mucosal secretory immunoglobulin A (SIgA) and serum immunoglobulin G (IgG) contents between the five groups (P>0.05). In summary, under the condition without attractant adding, the addition of rice extracting complex sugar to the diet of weaned piglets can improve the growth performance of piglets based on the methods of improving nutrient digestion, absorption, antioxidant capacity and intestinal barrier.
SAKATA T.Stimulatory effect of short-chain fatty acids on epithelial cell proliferation in the rat intestine:a possible explanation for trophic effects of fermentable fibre,gut microbes and luminal trophic factors[J].British Journal of Nutrition,1987,58(1):95-103.
[22]
KIM H S,KIM Y J,LEE H K,et al.Activation of macrophages by polysaccharide isolated from Paecilomyces cicadae through toll-like receptor 4[J].Food and Chemical Toxicology,2012,50(9):3190-3197.
[9]
LIU J B,CAO S C,LIU J,et al.Effect of probiotics and xylo-oligosaccharide supplementation on nutrient digestibility,intestinal health and noxious gas emission in weanling pigs[J].Asian-Australasian Journal of Animal Sciences,2018,31(10):1660-1669.
[18]
YIN J,REN W K,WU X S,et al.Oxidative stress-mediated signaling pathways:a review[J].Journal of Food Agriculture and Environment,2013,11(2):132-139.
SPREEUWENBERG M A M,VERDONK J M A J,GASKINS H R,et al.Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning[J].The Journal of Nutrition,2001,131(5):1520-1527.
PHILLIPS S M,STEPHENSON J R,AARONSON S A.Genetic factors infuencing mouse type-C RNA virus induction by naturally occurring B cell mitogens[J].Journal of Immunology,1977,118(2):662-666.
MCCRACKEN B A,SPURLOCK M E,ROOS M A,et al.Weaning anorexia may contribute to local inflammation in the piglet small intestine[J].The Journal of Nutrition,1999,129(3):613-619.
