Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep
WANG Chenchen, ZHANG Wenjie, HOU Chuanxiang, LI Xiaobin, ZHAO Guodong, YANG Kailun
Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China
Abstract:This experiment was conducted to investigate the effects of supplemented with different levels of equol on growth performance, plasma equol content and reproductive hormone levels and antioxidant capacity of female sheep. Twenty Kazakh female sheep with an average body weight of (36.73±2.42) kg, aged 2 years old, were randomly divided into 4 groups (5 sheep in each group), being control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ. Each sheep in all group was fed under the same feeding condition, additionally, sheep in trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed with 10, 25 and 50 mg equol at 08:00 every day per sheep, respectively. The supplementary feeding experiment lasted for 14 days. The results showed as follows:1) compared with control group, supplementation of 50 mg/(d·sheep) equol can significantly improve the average daily gain and average daily feed intake (P<0.05 or P<0.01), while reduce the feed/gain (P<0.05). 2) After different dosages of equol were supplementary fed, the plasma equol content tendency rising up at the beginning and declining in late, and it peaked around 2.0 to 3.0 h. 3) The plasma testosterone (T) level in trial group Ⅰ was significantly higher than that in control group (P<0.01) and trial group Ⅲ (P<0.05), and that in trial group Ⅱ was also significantly higher than that in control group (P<0.05), but there was no significant difference between trial group Ⅰ and trial group Ⅲ (P>0.05). The plasma dihydrotestosterone (DHT) level in trial group Ⅰ and trial group Ⅱ was significantly higher than that in control group and trial group Ⅲ (P<0.05). 4) The plasma total antioxidant capacity (T-AOC) and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in trial group Ⅱ had the highest values, while the malondialdehyde (MDA) content had the lowest value, and the differences in above indices were significant compared with control group (P<0.05 or P<0.01). Therefore, supplementation of 50 mg/(d·sheep) equol can significantly improve the growth performance of sheep; plasma equol content reaches its peak at 2.0 to 3.0 h after supplementary feeding equol, and with the supplemental dosage of equol increasing, the high equol content (i.e. duration time of high plasma equol content) prolongs in cross-peak time, too. Supplementation of 10 and 25 mg/(d·sheep) equol can significantly elevate the plasma T and DHT levels of female sheep. Supplementation of 25 mg/(d·sheep) equol can significantly enhance the antioxidant capacity of female sheep, and the effect is better than 10 and 50 mg/(d·sheep).
王晨晨, 张文杰, 侯传祥, 李晓斌, 赵国栋, 杨开伦. 补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响[J]. 动物营养学报, 2019, 31(8): 3801-3809.
WANG Chenchen, ZHANG Wenjie, HOU Chuanxiang, LI Xiaobin, ZHAO Guodong, YANG Kailun. Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep. Chinese Journal of Animal Nutrition, 2019, 31(8): 3801-3809.
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