补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响

doi:10.3969/j.issn.1006-267x.2019.08.042

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摘要本试验旨在研究补喂不同水平雌马酚对绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响。试验选取年龄为2岁、平均体重为（36.73±2.42）kg的健康、体况相近的哈萨克母羊20只，随机分为4组，分别为对照组、试验Ⅰ组、试验Ⅱ组、试验Ⅲ组，每组5只羊。各组试验羊在相同的饲养条件下饲养，在此基础上，试验Ⅰ组、试验Ⅱ组、试验Ⅲ组每日每只分别补喂10、25、50 mg雌马酚，进行为期14 d的饲养试验。结果表明：1）与对照组相比，每日每只母绵羊补喂50 mg雌马酚可以显著或极显著提高平均日增重、平均日采食量（P<0.05或P<0.01），显著降低料重比（P<0.05）。2）补喂雌马酚后血浆中雌马酚含量呈先上升后下降趋势，在2.0~3.0 h时达到峰值。3）试验Ⅰ组血浆中睾酮（T）水平显著或极显著高于对照组和试验Ⅲ组（P<0.05或P<0.01），试验Ⅱ组也显著高于对照组（P<0.05），但与试验Ⅰ组和试验Ⅲ组无显著差异（P>0.05）；试验Ⅰ组和试验Ⅱ组血浆中双氢睾酮（DHT）水平显著高于对照组和试验Ⅲ组（P<0.05）。4）血浆总抗氧化能力（T-AOC）与过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性均以试验Ⅱ组最高，丙二醛（MDA）含量以试验Ⅱ组最低，与对照组的差异均达到显著或极显著水平（P<0.05或P<0.01）。由此得出，在本试验条件下，每日给每只母绵羊补喂50 mg雌马酚可以显著提高绵羊的生长性能；补喂雌马酚后2.0~3.0 h，母绵羊血浆中雌马酚含量达到峰值，并且随着补喂剂量的提高，血浆中高含量雌马酚的跨峰时间（即维持血浆高含量雌马酚的持续时间）也延长。每日给每只母绵羊补喂10和25 mg雌马酚可显著提高血浆中T和DHT水平。每日给每只母绵羊补喂25 mg雌马酚可显著提高机体抗氧化能力，效果优于补喂10、50 mg时。。

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关键词 ：绵羊, 雌马酚, 生产性能, 生殖激素, 抗氧化能力

Abstract：This experiment was conducted to investigate the effects of supplemented with different levels of equol on growth performance, plasma equol content and reproductive hormone levels and antioxidant capacity of female sheep. Twenty Kazakh female sheep with an average body weight of (36.73±2.42) kg, aged 2 years old, were randomly divided into 4 groups (5 sheep in each group), being control group, trial group Ⅰ, trial group Ⅱ and trial group Ⅲ. Each sheep in all group was fed under the same feeding condition, additionally, sheep in trial group Ⅰ, trial group Ⅱ and trial group Ⅲ were fed with 10, 25 and 50 mg equol at 08:00 every day per sheep, respectively. The supplementary feeding experiment lasted for 14 days. The results showed as follows:1) compared with control group, supplementation of 50 mg/(d·sheep) equol can significantly improve the average daily gain and average daily feed intake (P<0.05 or P<0.01), while reduce the feed/gain (P<0.05). 2) After different dosages of equol were supplementary fed, the plasma equol content tendency rising up at the beginning and declining in late, and it peaked around 2.0 to 3.0 h. 3) The plasma testosterone (T) level in trial group Ⅰ was significantly higher than that in control group (P<0.01) and trial group Ⅲ (P<0.05), and that in trial group Ⅱ was also significantly higher than that in control group (P<0.05), but there was no significant difference between trial group Ⅰ and trial group Ⅲ (P>0.05). The plasma dihydrotestosterone (DHT) level in trial group Ⅰ and trial group Ⅱ was significantly higher than that in control group and trial group Ⅲ (P<0.05). 4) The plasma total antioxidant capacity (T-AOC) and the activities of catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in trial group Ⅱ had the highest values, while the malondialdehyde (MDA) content had the lowest value, and the differences in above indices were significant compared with control group (P<0.05 or P<0.01). Therefore, supplementation of 50 mg/(d·sheep) equol can significantly improve the growth performance of sheep; plasma equol content reaches its peak at 2.0 to 3.0 h after supplementary feeding equol, and with the supplemental dosage of equol increasing, the high equol content (i.e. duration time of high plasma equol content) prolongs in cross-peak time, too. Supplementation of 10 and 25 mg/(d·sheep) equol can significantly elevate the plasma T and DHT levels of female sheep. Supplementation of 25 mg/(d·sheep) equol can significantly enhance the antioxidant capacity of female sheep, and the effect is better than 10 and 50 mg/(d·sheep).

Key words： sheep equol growth performance reproductive hormones antioxidant capacity

收稿日期: 2019-01-21

PACS:

S826

基金资助:新疆维吾尔自治区重点实验室开放课题“雌马酚高效转化细菌筛选及富含雌马酚牛乳生产的研究”（2016D03012）

通讯作者: 杨开伦,教授,博士生导师,E-mail:yangkailun2002@aliyun.com E-mail: yangkailun2002@aliyun.com

作者简介: 王晨晨(1993-),女,新疆乌苏人,硕士研究生,研究方向为草食动物营养代谢。E-mail:2225927653@qq.com

引用本文:

王晨晨, 张文杰, 侯传祥, 李晓斌, 赵国栋, 杨开伦. 补喂不同水平雌马酚对母绵羊生长性能、血浆中雌马酚含量与生殖激素水平以及抗氧化能力的影响[J]. 动物营养学报, 2019, 31(8): 3801-3809.
WANG Chenchen, ZHANG Wenjie, HOU Chuanxiang, LI Xiaobin, ZHAO Guodong, YANG Kailun. Effects of Supplemented with Different Levels of Equol on Growth Performance, Plasma Equol Content and Reproductive Hormone Levels and Antioxidant Capacity of Female Sheep. Chinese Journal of Animal Nutrition, 2019, 31(8): 3801-3809.

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http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2019.08.042 或 http://www.chinajan.com/CN/Y2019/V31/I8/3801

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