Abstract：The purpose of this study was to investigate the changes of key genes of Toll-like receptor 4 (TLR4) and nucleotide-bound oligomeric domain receptor (NOD) inflammation signaling pathway in spleen of weaned piglets at different time after lipopolysaccharide (LPS) challenge. Forty-two Duroc×Landrace×Yorkshine weaned piglets with a body weight of (7.1±0.9) kg were selected and randomly divided into 7 treatments (6 pigs per treatment). The piglets were injected with 100 μg/kg body weight LPS, and slaughtered at 0 h (before LPS challenge), 1, 2, 4, 8, 12 and 24 h (after LPS challenge). Blood and spleen samples were taken to determine the blood cell differential counts and the mRNA expressions of inflammatory related genes, respectively. The results showed as follows:LPS challenge led to significant changes in blood cell classification count (P<0.05). The mRNA expressions of key genes of TLR4 and NOD signaling pathway were increased after LPS challenge (P<0.05), and reached the peak at 2 to 8 h. The mRNA expressions of tumor necrosis factor-α, interleukin-1β, interleukin-6, cyclooxygenase 2 and heat shock protein 70 were also significantly increased after LPS challenge (P<0.05), and peaked at 1 to 4 h. At 8 to 24 h post injection, these genes gradually returned to normal level or slightly lower than normal level. Overall, LPS challenge activates the inflammatory signaling pathway and up-regulates the expression of inflammatory cytokines. During LPS stimulation, the expressions of these gene increase at the early time, and then decrease gradually after the peak.
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WANG Yang, LI Xiangen, LIU Yulan, ZHU Huiling, WANG Xiuying. Effects of Lipopolysaccharide Challenge on Key Genes Expression of Inflammatory Signaling Pathway in Spleen of Weaned Piglets. Chinese Journal of Animal Nutrition, 2019, 31(8): 3882-3890.
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