Utilization of Methionine by Rumen Microorganisms and Characteristics of Microbial Nitrogen Transfer
REN Ao1,2, ZHU Jinjia1,2, CHEN Liang1,2, KONG Zhiwei2, LIU Qian2, GUO Yibing2,3, XU Qixiang2, ZHOU Chuanshe1,2, ZHANG Bin1, TAN Zhiliang2
1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Institute of Subtropical Agricultural Ecology, Chinese Academy of Sciences, Changsha 410125, China;
3. College of Animal Science, Southwest University, Chongqing 402460, China
Abstract：The aim of this study was to investigate the utilization of methionine (Met) by rumen microorganisms and effects of Met on microbial nitrogen transfer. Corn-soybean meal concentrate and (0.5 g) and corn-straw diet (0.5 g) were used for fermentation substrates, the study was conducted as one-factor block experimental design, and set up test group and control group. The test group was supplemented with 0.33 μmol isotope labeling 13C methionine (13C-Met,addition of 0.18 mL solution to in vitro culture substrate), and the control group was supplemented with equivalent distilled water, to explore the effects of Met on in vitro fermentation pH, the contents of ammonia nitrogen (NH3-N), volatile fatty acid (VFA), microbial nitrogen and microbial Met and the abundance of 13C in microbial. The results showed as follows:1) adding Met could significantly increase the pH of fermentation liquid (P<0.01); 2) adding Met could significantly increase the abundance of 13C in microorganisms (P<0.01); 3) adding Met had no significant effects on the contents of NH3-N, VFA, microbial nitrogen and microbial Met (P>0.05). Therefore, it can be inferred that rumen microorganisms can effectively utilize Met, Met has some influence on fermentation characteristics, but do not affect the flow of microbial nitrogen.
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