Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte
YU Chunwei1, LI Dongfang1, LIU Shixiong1, HAO Lingkui1, GAO Min2, HU Honglian2, LIU Dacheng1
1. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Research Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Animal Sciences, Hohhot 010031, China
Abstract：This experiment was conducted to study the effects of β-glucan from Saccharomyces cerevisiae on the antioxidant capacity of sheep lymphocytes. Based on the establishment of lipopolysaccharide (LPS)-induced oxidative stress in sheep lymphocytes, the effects of β-glucan on cell viability were determined by MTT assay by adding different concentrations [0(control), 5, 10, 20, 50 and 100 μg/mL] of β-glucan to the culture medium. The biochemical method was used to determine the effects of β-glucan on the changes of antioxidant indexes in sheep lymphocytes. The results showed as follows: β-glucan showed no cytotoxicity after being applied to lymphocytes at a concentration of 0 to 20 μg/mL, when the concentration of β-glucan was 50 μg/mL, the cell viability decreased significantly (P<0.05); compared with the control group, the malonaldehyde (MDA) content extremely significantly increased (P<0.01), superoxide dismutase (ROS) and catalase (CAT) activities extremely significantly decreased (P<0.05), and glutathion peroxidase (GSH-Px) activity decreased extremely significantly (P<0.01) in lymphocytes treated by LPS. After adding 10 μg/mL β-glucan for 6 h, compared with the LPS group, the activity of SOD and CAT in lymphocytes extremely significantly increased (P<0.01), the activity of GSH-Px significantly increased (P<0.05), and the contents of MDA and ROS extremely significantly decreased (P<0.01). In conclusions, LPS can induce oxidative stress in sheep lymphocytes. β-glucan can inhibit LPS-induced oxidative injury of lymphocytes and has a good protective effect on LPS-induced oxidative injury of sheep lymphocytes, and there is a dose-dependent effect.
于春微, 李冬芳, 刘世雄, 郝凌魁, 高民, 胡红莲, 刘大程. 酵母β-葡聚糖对脂多糖诱导的绵羊淋巴细胞氧化应激损伤的保护作用[J]. 动物营养学报, 2020, 32(6): 2904-2910.
YU Chunwei, LI Dongfang, LIU Shixiong, HAO Lingkui, GAO Min, HU Honglian, LIU Dacheng. Protective Effects of β-Glucan from Saccharomyces cerevisiae on Lipopolysaccharide Induced Oxidative Stress Injury in Sheep Lymphocyte. Chinese Journal of Animal Nutrition, 2020, 32(6): 2904-2910.
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