Effects of Early Weaning on Expression of Glutamate/Glutamine Transporters in Small Intestine of Piglets
WANG Qiuju1, CUI Yizhe1, WANG Mengzhu1, JIA Junfeng1, HU Haiyan2, WU Zhimin2, GENG Zhongcheng1
1. College of Animal Science and Veterinary, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Animal Husbandry Bureau of Duerbert Mongolia Autonomous County, Daqing 166299, China
Abstract：The purpose of this study was to study the effects of early weaning on the expression of glutamate/glutamine transporters in small intestine of piglets. A total of 40 piglets with similar body weight and 10 days of age were selected from 40 piglets of different sows. They were randomly divided into 2 groups with 20 piglets in each group. Control group piglets were suckling piglets, feeding with sows. The experimental group piglets were weaning piglets, isolated and weaned. The trial lasted for 10 days. At the end of feeding, 12 piglets were randomly selected from each group, jejunum and ileum were collected, and the gene and protein expression of glutamate/glutamine transporter were determined. The results showed as follows:compared with suckling piglets, early weaning significantly increased the expression of glutamate/cystine exchange transporter (xCT) and neutral amino acid transporter 2(ASCT2) proteins and mRNA in the jejunum and ileum of the piglets (P<0.05). Pearson correlation analysis results showed that the expression levels of xCT and ASCT2 proteins in issues homogenate, cell endoplasm, apical membrane of weaning piglets and suckling piglets showed a positive linear relationship with the expression levels of corresponding mRNA in jejunum and ileum (P<0.05). The height of villi in the jejunum and ileum of weaning piglets decreased, and the crypt depth increased, with significant differences (P<0.05). The total antioxidant capacity in jejunum and ileum of weaning piglets significantly reduced (P<0.05), and the content of oxidized glutathione (GSSG) in jejunum significantly increased (P<0.05), and the content of glutathione (GSH) significantly decreased in ileum (P<0.05). Early weaning places the small intestine of piglets in a state of oxidative stress, significantly increases the expression levels of xCT and ASCT2 proteins and their mRNA in the small intestinal tissues of weaning piglets, so as to promote the uptake of glutamate/glutamine and reduce the level of oxidative stress in the small intestine of piglets.
王秋菊, 崔一喆, 王梦竹, 贾军峰, 胡海燕, 武志敏, 耿忠诚. 早期断奶对仔猪小肠中谷氨酸/谷氨酰胺转运载体表达的影响[J]. 动物营养学报, 2020, 32(7): 3324-3332.
WANG Qiuju, CUI Yizhe, WANG Mengzhu, JIA Junfeng, HU Haiyan, WU Zhimin, GENG Zhongcheng. Effects of Early Weaning on Expression of Glutamate/Glutamine Transporters in Small Intestine of Piglets. Chinese Journal of Animal Nutrition, 2020, 32(7): 3324-3332.
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