Abstract：The mammary gland is composed of acini with lactation function. Mammary epithelial cells (MECs) are arranged in a single layer around the acinus, which is an important part of the innate immune barrier of the mammary gland. It is also responsible for transforming nutrients in the blood into milk through a series of complex biochemical processes. The separation and culture of bovine mammary epithelial cells (BMECs) in vitro can solve many problems, such as uncontrollable conditions, difficult operation, high cost and large individual differences in vivo test, and also provide a good cell model for in vitro study of the growth and development of mammary tissue, the mechanism of lactation and breast diseases. This paper mainly summarized the classification and function of BMECs, culture methods, mode and their application in gene expression mechanism and omics, in order to provide valuable reference and new ideas for the in vitro culture and related research of BMECs.
母童, 虎红红, 顾亚玲, 张娟. 牛乳腺上皮细胞体外分离培养模式、方法及其应用进展[J]. 动物营养学报, 2021, 33(1): 56-67.
MU Tong, HU Honghong, GU Yaling, ZHANG Juan. Isolation and Culture Mode, Method and Application of Bovine Mammary Epithelial Cells in Vitro. Chinese Journal of Animal Nutrition, 2021, 33(1): 56-67.
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