Effects of Compound Probiotics Solid-State Fermented Soybean Meal on Growth Performance, Intestinal Morphology and Intestinal Microbiota of Weaned Piglets
XIONG Yunxia, ZHANG Yahui, LI Ping, WU Qiwen, QIU Yueqin, YI Hongbo, XIAO Hao, JIANG Zongyong, WANG Li
State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of Agriculture, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Abstract：The objective of this study was to investigate the effects of self-made compound probiotics fermented soybean meal (FSBM) on the growth performance, intestinal morphology and intestinal microbiota of weaned piglets. A total of 72 three line hybrid (Duroc×Landrace×Yorkshire) weaned piglets at 21 days of age with initial body weight of (7.49±0.73) kg were randomly divided into two groups, 6 repeats per group and 6 piglets per repeat (half male and half female), respectively. The control group (CON group) fed a corn-soybean (9% soybean meal) meal without antibiotics, and the FSBM group fed a self-made compound probiotics solid-state FSBM replaced the components of soybean meal in the control group. The experiment lasted for 14 days. The initial and final body weight, and diarrhea of piglets, and feed intake of repeats were accurately recorded. Samples of duodenum, jejunum and ileum were taken for staining to measure intestinal villi height and crypt depth, and for real time PCR to determine the mRNA relative expression levels of absorption-related transporters. The contents of cecum were collected, and the changes of intestinal microbiota were analyzed by 16S rRNA sequencing (V3+V4). The results showed as follows: compared with control group, 1) adding FSBM could significantly elevate average daily gain (ADG) (P<0.05), and significantly reduced the ratio of feed to gain (P<0.05); 2) adding FSBM could significantly improve the villi height of jejunum and the crept depth of duodenum (P<0.05); 3) adding FSBM could significantly increase the mRNA relative expression levels of sodium-dependent glucose co-transporter 1 (SGLT1) in duodenum, SGLT1, cationic amino acid transporter (b0,+AT) mRNA in jejunum and small peptide transporter 1 (PepT1) and aquaporin 1 (AQP1) in ileum (P<0.05); 4) adding FSBM showed a tendency to increase the diversity and richness of intestinal flora, and Clostridia, Clostridiales, Lachnospiraceae, Muribaculaceae, Turicibacter and Lactobacillus mucosae were enriched in FSBM group. In conclusion, using the self-made FSBM to replace the content of soybean meal in diet (9%) can significantly improve the growth performance of weaned piglets, improves the intestinal morphology and absorption function, and has a tendency to increase the diversity of intestinal microbiota.
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