Main Factors Affecting Porcine Intermuscular Fat Deposition and Potential Regulation Mechanism
YANG Xizi1, HU Ruizhi1, HE Jianhua1, WU Shusong1,2
1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Hunan Co-Innovation Center of Safety Animal Production, Changsha 410128, China
Abstract：Fat deposition, especially intermuscular fat deposition, is closely related to the pork quality. Genetics, nutrition and environment are considered to be the main factors affecting fat deposition, although the mechanisms are not clear. Based on previous studies, this paper has summarized the differences in fat deposition between species and individual parts, the regulatory effects of genes, proteins and enzymes in the major signaling pathways affecting fat deposition on fat oxidation and synthesis, as well as the effects of nutrition on intermuscular fat deposition and meat quality, in order to provide reference for the research and application of improving pork quality by regulating intermuscular fat depositon.
李琳,郑卓,贺红专.PPARγ基因调控猪脂肪沉积研究进展[J].中国猪业,2016,11(8):57-60. LI L,ZHENG Z,HE H Z.Research progress of PPARγ gene regulation of pig fat deposition[J].China Swine Industry,2016,11(8):57-60.(in Chinese)
[2]
MOTTRAM D S.Flavour formation in meat and meat products:a review[J].Food Chemistry,1998,62(4):415-424.
[1]
韩磊,王术坤.2019年中国猪肉供需形势及2020年展望[J].农业展望,2020,16(4):7-11,17. HAN L,WANG S K.Situation of China's pork supply and demand in 2019 and its prospect in 2020[J].Agricultural Outlook,2020,16(4):7-11,17.(in Chinese)
[31]
郭玉婷,王智慧,刘星彤,等.AMPK在肥胖中的研究进展[J].吉林医药学院学报,2020,41(4):306-309. GUO Y T,WANG Z H,LIU X T,et al.Research progress of AMPK in obesity[J].Journal of Jilin Medical University,2020,41(4):306-309.(in Chinese)
[24]
ZHANG S H,SHEN L Y,XIA Y D,et al.DNA methylation landscape of fat deposits and fatty acid composition in obese and lean pigs[J].Scientific Reports,2016,6:35063.
[50]
张艳芳.CPT1基因对猪脂肪沉积的影响及其调控机制的研究[D].博士学位论文.杭州:浙江大学,2010. ZHANG Y F.Effect of CPT1 gene on porcine fat deposition and its molecular mechanism[D].Ph.D.Thesis.Hangzhou:Zhejiang University,2010.(in Chinese)
[54]
GNIULI D,ROSA G,MANCO M,et al.Changes in fat mass influence SREBP-1c and UCP-2 gene expression in formerly obese subjects[J].Obesity,2012,13(3):567-573.
[56]
GOSMAIN Y,DIF N,BERBE V,et al.Regulation of SREBP-1 expression and transcriptional action on HKⅡ and FAS genes during fasting and refeeding in rat tissues[J].Journal of Lipid Research,2005,46(4):697-705.
[60]
FREYTAG S O,PAIELLI D L,GILBERT J D.Ectopic expression of the CCAAT/enhancer-binding protein alpha promotes the adipogenic program in a variety of mouse fibroblastic cells[J].Genes & Development,1994,8(14):1654-1663.
[84]
CORDERO G,ISABEL B,MENOYO D,et al.Dietary CLA alters intramuscular fat and fatty acid composition of pig skeletal muscle and subcutaneous adipose tissue[J].Meat Science,2010,85(2):235-239.
[86]
陈代文,李永义,张克英,等.饲粮添加高铜和VE对猪生长性能和猪肉品质的影响[J].四川农业大学学报,2002,20(1):23-28. HEN D W,LI Y Y,ZHANG K Y,et al.Growth performance and meat quality of pigs fed diets supplemented with or without 250 mg/kg copper and 200 mg/kg vitamin E[J].Journal of Sichuan Agricultural University,2002,20(1):23-28.(in Chinese)
[90]
COSTA C D S,ROHDEN F,HAMMES T O,et al.Resveratrol upregulated SIRT1,FOXO1,and adiponectin and downregulated PPARγ1-3 mRNA expression in human visceral adipocytes[J].Obesity Surgery,2011,21(3):356-361.
[4]
MA J D,YU S Z,WANG F J,et al.MicroRNA transcriptomes relate intermuscular adipose tissue to metabolic risk[J].International Journal of Molecular Sciences,2013,14(4):8611-8624.
[6]
李鹏,斯日古楞.营养调控影响猪肌内脂肪沉积的研究进展[J].饲料广角,2010(22):34-35. LI P,SIRIGULENG.Research progress on nutritional regulation affecting pig intramuscular fat deposition[J].Feed China,2010(22):34-35.(in Chinese)
[10]
KOUBA M,SELLIER P.A review of the factors influencing the development of intermuscular adipose tissue in the growing pig[J].Meat Science,2011,88(2):213-220.
[14]
CAMERON N D,ENSER M,NUTE G R,et al.Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat[J].Meat Science,2000,55(2):187-195.
[16]
FONTANESI L,SCHIAVO G,GALIMBERTI G,et al.A genome-wide association study for a proxy of intermuscular fat level in the Italian Large White breed identifies genomic regions affecting an important quality parameter for dry-cured hams[J].Animal Genetics,2017,48(4):459-465.
[20]
苗志国,谢红兵,王永强,等.淮南猪与长白猪脂肪代谢酶及脂联素的发育差异[J].贵州农业科学,2018,46(1):61-63. MIAO Z G,XIE H B,WANG Y Q,et al.Development defference in fat metabolic enzymes and serum adiponectin between Huainan and Landrace pig[J].Guizhou Agricultural Sciences,2018,46(1):61-63.(in Chinese)
[26]
GE Z J,LUO S M,LIN F,et al.DNA Methylation in oocytes and liver of female mice and their offspring:effects of high-fat-diet-induced obesity[J].Environmental Health Perspectives,2014,122(2):159-164.
[30]
CARLING D.The AMP-activated protein kinase cascade-a unifying system for energy control[J].Trends in Biochemical Sciences,2004,29(1):18-24.
[34]
LIU B,YANG T,LUO Y,et al.Oat β-glucan inhibits adipogenesis and hepatic steatosis in high fat diet-induced hyperlipidemic mice via AMPK signaling[J].Journal of Functional Foods,2018,41:72-82.
[44]
MERRILL G F,KURTH E J,HARDIE D G,et al.AICA riboside increases AMP-activated protein kinase,fatty acid oxidation,and glucose uptake in rat muscle[J].The American Journal of Physiology,1997,273(6):E1107-E1112.
[7]
王琤韡,王仁华.猪肉肌间脂肪的营养调控[J].中国饲料,2015(24):29-31. WANG Z W,WANG R H.Nutritional regulation of pork intermuscular fat[J].China Feed,2015(24):29-31.(in Chinese)
[11]
郑梦奇,石放雄.生长猪肌间脂肪组织发育的生物学特性及其影响因素[J].猪业科学,2011,28(10):24-26. ZHENG M Q,SHI F X.Biological characteristics and influencing factors of intermuscular adipose tissue development in growing pigs[J].Swine Industry Science,2011,28(10):24-26.(in Chinese)
[17]
KOUBA M,BONNEAU M,NOBLET J.Relative development of subcutaneous,intermuscular,and kidney fat in growing pigs with different body compositions[J].Journal of Animal Science,1999,77(3):622-629.
[21]
WOOD J D,BROWN S N,NUTE G R,et al.Effects of breed,feed level and conditioning time on the tenderness of pork[J].Meat Science,1996,44(1/2):105-112.
[27]
LI M Z,WANG T,WU H L,et al.Genome-wide DNA methylation changes between the superficial and deep backfat tissues of the pig[J].International Journal of Molecular Sciences,2012,13(6):7098-7108.
[37]
PARK S,JI Y,JUNG H Y,et al.Lactobacillus plantarum HAC01 regulates gut microbiota and adipose tissue accumulation in a diet-induced obesity murine model[J].Applied Microbiology and Biotechnology,2017,101(4):1605-1614.
[41]
李优磊.PPAR信号通路在调控猪皮下脂肪与肌内脂肪差异沉积中的作用及机制研究[D].博士学位论文.杨凌:西北农林科技大学,2018. LI Y L.The defferential function and mechanism of PPAR signaling in regulation of porcine subcutaneous and intramuscular fat deposition[D].Ph.D.Thesis.Yangling:Northwest A & F University,2018.(in Chinese)
[5]
罗嘉,蒲强,余霞,等.猪肌内脂肪沉积机理研究进展[J].猪业科学,2014,31(10):114-116. LUO J,PU Q,YU X,et al.Research progress on the mechanism of intramuscular fat deposition in pigs[J].Swine Industry Science,2014,31(10):114-116.(in Chinese)
[15]
JANKOWIAK H,BOCIAN M,BARCZAK J.The effect of intramuscular fat content on the meat quality of PLW×PL pigs[J].Italian Journal of Food Science,2019,31(1):87-97.
[25]
邓洛娜,罗祖朋,张志望,等.不同猪种脂肪组织基因组DNA甲基化分析[J].基因组学与应用生物学,2018,37(11):4730-4735. DENG L N,LUO Z P,ZHANG Z W,et al.Analysis of DNA methylation in adipose tissues genomes of different pig breeds[J].Genomics and Applied Biology,2018,37(11):4730-4735.(in Chinese)
[35]
YAO C G,PANG D X,LU C,et al.Data mining and validation of AMPK pathway as a novel candidate role affecting intramuscular fat content in pigs[J].Animals (Basel),2019,9(4):137.
[45]
张幸开.维生素A对肉牛肌内脂肪沉积及ACC/HSL、PPARγ基因表达的影响[D].硕士学位论文.杨凌:西北农林科技大学,2005. ZAHNG X K.The influence of intramuscular fat deposit and gene expression of ACC/HSL and PPARγ with the supplemetation of vitamin A in beef cattle[D].Master's Thesis Yangling:Northwest A & F University,2005.(in Chinese)
[47]
王斐.二甲双胍对高脂饲养大鼠肝脏AMPK和PPARs活性影响——改善脂肪肝的可能机制[D].博士学位论文.济南:山东大学,2008. WANG F.Effects of metformin on the activities of hepatic AMPK and PPARs in high-fat fed rat:possible mechanism of ameliroting hepatic steatosis[D].Ph.D.Thesis.Jinan:Shandong University,2008.(in Chinese)
[51]
郝梦娇,党院霞,周欣欣,等.黄芪散对高脂血症大鼠AMPK/ACC/CPT1通路的影响[J].中国中药杂志,2018,43(12):2586-2592. HAO M J,DANG Y X,ZHOU X X,et al.Effect of Huangqi San on AMPK/ACC/CPT1 pathway in hyperlipidemia rats[J].China Journal of Chinese Materia Medica,2018,43(12):2586-2592.(in Chinese)
[55]
KIM J B,SPIEGELMAN B M.ADD1/SREBP1 promotes adipocyte differentiation and gene expression linked to fatty acid metabolism[J].Genes & Development,1996,10(9):1096-1107.
[57]
王寒凝,祁智,李雪玲,等.肌内脂肪沉积的营养调控与分子机制[J].动物营养学报,2020,32(7):2947-2958. WANG H N,QI Z,LI X L,et al.Nutritional regulation and molecular mechanism of intramuscular fat deposition[J].Chinese Journal of Animal Nutrition,2020,32(7):2947-2958.(in Chinese)
[61]
HE Y F,LIU F Y,ZHANG W X.Tangeritin inhibits adipogenesis by down-regulating C/EBPα,C/EBPβ,and PPARγ expression in 3T3-L1 fat cells[J].Genetics and Molecular Research,2015,14(4):13642-13648.
[3]
AKHMEDKHANOV A,ZELENIUCH-JACQUOTTE A,TONIOLO P.Role of exogenous and endogenous hormones in endometrial cancer:review of the evidence and research perspectives[J].Annals of the New York Academy of Sciences,2001,943(1):296-315.
[8]
章杰,刘一辉,李学伟.影响猪肌间脂肪组织发育的因素[J].养猪,2013(6):60-64. ZHANG J,LIU Y H,LI X W.Factors affecting the development of intermuscular adipose tissue in pigs[J].Swine Production,2013(6):60-64.(in Chinese)
[12]
KOUBA M,BONNEAU M.Compared development of intermuscular and subcutaneous fat in carcass and primal cuts of growing pigs from 30 to 140 kg body weight[J].Meat Science,2009,81(1):270-274.
[13]
马佩云.JAK2/STAT3通路对小鼠骨骼肌发育、能量代谢及肌间脂肪相关基因表达的影响[D].硕士学位论文.杨凌:西北农林科技大学,2010. MA P Y.The effect of JAK2/STAT3 signal pathway on the expression of skeletal muscle development and energy metabolic related genes in mice[D].Master's Thesis.Yangling:Northwest A & F University,2010.(in Chinese)
[18]
李盼,赵雨农,段梦琪,等.不同品种猪SCD基因在肝脏、背脂、背最长肌中表达差异研究[J].高原农业,2019,3(5):528-532,538. LI P,ZHAO Y N,DUAN M Q,et al.Differential expression of SCD gene in different breeds of pigs in liver,back fat and longissimus dorsi muscle[J].Journal of Flateau Agriculture,2019,3(5):528-532,538.(in Chinese)
[19]
陶璇,顾以韧,梁艳,等.藏猪和杜洛克猪SCD、CPT1B基因表达差异及其与IMF含量相关性分析[J].黑龙江畜牧兽医,2017(13):82-85. TAO X,GU Y R,LIANG Y,et al.Differential expression of SCD and CPT1B genes and their correlation analysis with IMF contents in Tibet and Duroc pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2017(13):82-85.(in Chinese)
[22]
KULIS M,QUEIRÓS A C,BEEKMAN R,et al.Intragenic DNA methylation in transcriptional regulation,normal differentiation and cancer[J].Biochimica et Biophysica Acta:Gene Regulatory Mechanisms,2013,1829(11):1161-1174.
[23]
王鹤洁,秦本源,王媛媛,等.DNA甲基化与去甲基化调控脂肪沉积的研究进展[J].中国畜牧兽医,2019,46(2):519-525. WANG H J,QIN B Y,WANG Y Y,et al.Research advances on DNA methylation and demethylation to regulate adipose deposition[J].China Animal Husbandry and Veterinary Medicine,2019,46(2):519-525.(in Chinese)
[28]
CALVO L,SEGURA J,TOLDRÁ F,et al.Meat quality,free fatty acid concentration,and oxidative stability of pork from animals fed diets containing different sources of selenium[J].Food Science and Technology International,2017,23(8):716-728.
[29]
OSLER M E,ZIERATH J R.Minireview:adenosine 5'-monophosphate-activated protein kinase regulation of fatty acid oxidation in skeletal muscle[J].Endocrinology,2008,149(3):935-941.
[32]
HAWLEY S A,DAVISON M,WOODS A,et al.Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase[J].The Journal of Biological Chemistry,1996,271(44):27879-27887.
[33]
LAGE R,DIÉGUEZ C,VIDAL-PUIG A,et al.AMPK:a metabolic gauge regulating whole-body energy homeostasis[J].Trends in Molecular Medicine,2008,14(12):539-549.
[36]
VAEZI M,YAGHMAEI P,HAYATI-ROODBARI N,et al.Citral effect in male NMRI mice nonalcoholic steatosis model:assessing biochemical and histological parameters and PPARα gene expression[J].Brazilian Journal of Pharmaceutical Sciences,2018,54(3):11.
[38]
高倩.PPARα信号通路在小鼠脂肪肝形成过程中的作用研究[D].博士学位论文.杨凌:西北农林科技大学,2016. GAO Q.Roles of PPARα signaling pathway in the development of fatty liver in mice[D].Ph.D.Thesis.Yangling:Northwest A & F University,2016.(in Chinese)
[39]
CAI H Y,WEN Z G,LI X M,et al.Lactobacillus plantarum FRT10 alleviated high-fat diet-induced obesity in mice through regulating the PPARα signal pathway and gut microbiota[J].Applied Microbiology and Biotechnology,2020,104(4):5959-5972.
[40]
YE J M,DOYLE P J,IGLESIAS M A,et al.Peroxisome proliferator-activated receptor (PPAR)-α activation lowers muscle lipids and improves insulin sensitivity in high fat-fed rats:comparison with PPAR-γ activation[J].Diabetes,2001,50(2):411-417.
[42]
CUI J X,CHEN W,LIU J,et al.Study on quantitative expression of PPARγ and ADRP in muscle and its association with intramuscular fat deposition of pig[J].Springerplus,2016,5(1):1501.
[43]
丛金凤,范颖,李新,等.基于AMPK/SREBP1信号通路探究黄芪葛根汤有效组分对糖尿病大鼠脂代谢及炎症反应的作用机制[J/OL].中华中医药学刊,2020:1-9.[2020-07-12].http://kns.cnki.net/kcms/detail/21.1546.R.20200702.1439.092.html. CONG J F,FAN Y,LI S,et al.Based on AMPK/SREBP1 signaling pathway,to explore the mechanism of the effective components of Haungqi Gegen decoction to lipids metabolism and inflammatory response in diabetic rats[J/OL].Chinese Archives of Traditional Chines Medicine:1-9.[2020-07-12].http://kns.cnki.net/kcms/detail/21.1546.R.20200702.1439.092.html.
[46]
UNDERWOOD K R,MEANS W J,ZHU M J,et al.AMP-activated protein kinase is negatively associated with intramuscular fat content in longissimus dorsi muscle of beef cattle[J].Meat Science,2008,79(2):394-402.
[48]
苏东玮,皮浩,方国恩,等.低糖低氧状态下AMPK通路通过PPARα调控CPT1c影响人甲状腺乳头状癌B-CPAP细胞的凋亡[J].中国肿瘤生物治疗杂志,2020,27(5):508-514. SU D W,PI H,FANG G E,et al.Effect of AMPK pathway on apoptosis of human thyroid papillary cancer B-CPAP cells under low glucose and hypoxia conditions through CPT1c regulated by PPARα[J].Chinese Journal of Cancer Biotherapy,2020,27(5):508-514.(in Chinese)
[49]
WAKIL S J,ABU-ELHEIGA L A.Fatty acid metabolism:target for metabolic syndrome[J].Journal of Lipid Research,2009,50(Suppl.1):S138-S143.
[52]
徐丽.牛SREBP1基因与牛肉肌内脂肪沉积的研究[D].硕士学位论文.北京:中国农业科学院,2013. XU L.Study on beef intramuscular fat deposition of SREBP1 gene in cattle[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2013.(in Chinese)
[65]
LOMAX M A,KARAMANLIDIS G,LAWS J,et al.Pigs fed saturated fat/cholesterol have a blunted hypothalamic-pituitary-adrenal function,are insulin resistant and have decreased expression of IRS-1,PGC1α and PPARα[J].The Journal of Nutritional Biochemistry,2013,24(4):656-663.
[67]
LI Y H,LI F N,WU L,et al.Effects of dietary protein restriction on muscle fiber characteristics and mTORC1 pathway in the skeletal muscle of growing-finishing pigs[J].Journal of Animal Science and Biotechnology,2016,7(1):47.
[71]
徐林,单安山,张宏宇.母猪哺乳期蛋白限饲对后代肉质性状及肌纤维发育的影响[J].东北农业大学学报,2011,42(6):12-17. XU L,SHAN A S,ZHANG H Y.Effects of protein restriction in sows during lactation on meat quality traits and muscle fiber in offspring[J].Journal of Northeast Agricultural University,2011,42(6):12-17.(in Chinese)
[75]
HE Q H,KONG X F,WU G Y,et al.Metabolomic analysis of the response of growing pigs to dietary L-arginine supplementation[J].Amino Acids,2009,37(1):199-208.
[77]
马现永,蒋宗勇,林映才,等.精氨酸对肥育猪肌内脂肪含量的影响及其机理研究[C]//第六次全国饲料营养学术研讨会论文集.杨凌:中国畜牧兽医学会,2010:367. MA X Y,JIANG Z Y,LING Y C,et al.Effect of arginine on intramuscular fat content and its mechanism[C]//Proceedings of the Sixth National Symposium on Feed Nutrition.Yangling:Chinese Association of Animal Science and Veterinary Medicine,2010:367.(in Chinese)
[81]
THIEL-COOPER R L,PARRISH F C,Jr.,SPARKS J C,et al.Conjugated linoleic acid changes swine performance and carcass composition[J].Journal of Animal Science,2001,79(7):1821-1828.
[85]
ZHONG W J,JIANG Z Y,ZHENG C T,et al.Relationship between proteome changes of Longissimus muscle and intramuscular fat content in finishing pigs fed conjugated linoleic acid[J].The British Journal of Nutrition,2011,105(1):1-9.
[87]
PARK J B,LEEH S,HWANG S Y,et al.Effect of combined plant extracts on anti-obesity in rats fed a high-fat diet[J].Asian Journal of Beauty & Cosmetology,2011,9(1):25-34.
[53]
YAHAGI N,SHIMANO H,HASTY A H,et al.Absence of sterol regulatory element-binding protein-1(SREBP-1) ameliorates fatty livers but not obesity or insulin resistance in Lepob/Lepob mice[J].The Journal of Biological Chemistry,2002,277(22):19353-19357.
[63]
单体中,汪以真.畜禽脂肪酸结合蛋白基因研究进展[J].中国饲料,2006(19):27-28. SHAN T Z,WANG Y Z.Research progress of fatty acid binding protein gene in livestock and poultry[J].China Feed,2006(19):27-28.(in Chinese)
[73]
郑昌炳,段叶辉,邓近平,等.氨基酸调控猪肉品质的研究进展[J].动物营养学报,2020,32(7):2975-2980. ZHENG C B,DUAN Y H,DENG J P,et al.Research progress of amino acid on nutrition regulation of pork quality[J].Chinese Journal of Animal Nutrition,2020,32(7):2975-2980.(in Chinese)
[83]
TOUS N,THEIL P K,LAURIDSEN C,et al.Dietary conjugated linoleic acid modify gene expression in liver,muscles,and fat tissues of finishing pigs[J].Journal of Animal Science,2012,90(Suppl. 4):340-342.
[58]
ZHANG L,HUANG Y,LIU F,et al.Vanadium(Ⅳ)-chlorodipicolinate inhibits 3T3-L1 preadipocyte adipogenesis by activating LKB1/AMPK signaling pathway[J].Journal of Inorganic Biochemistry,2016,162:1-8.
[59]
刘毅,娄少颖,何燕铭,等.小檗碱对3T3-L1前脂肪细胞增殖及分化相关基因PPARγ、C/EBPα mRNA和蛋白表达的影响[J].中国中西医结合杂志,2008,28(11):1005-1009. LIU Y,LOU S Y,HE Y M,et al.Effects of berberine on cell proliferation,peroxisome proliferation activated receptor γ,CAAT/enhancer binding protein mRNA and protein expression in 3T3-L1 pre-adipocytes[J].Chinese Journal of Integrated Traditional and Western Medicine,2008,(11):1005-1009.(in Chinese)
[69]
WOOD J D,NUTE G R,RICHARDSON R I,et al.Effects of breed,diet and muscle on fat deposition and eating quality in pigs[J].Meat Science,2004,67(4):651-667.
[79]
马清泉,王国红,周昕博,等.亮氨酸和异亮氨酸对脂肪沉积的影响及机制[J].东北农业大学学报,2020,51(1):50-56,64. MA Q Q,WANG G H,ZHOU X B,et al.Effect and mechanism of leucine and isoleucine on fat deposition[J].Journal of Northeast Agricultural University,2020,51(1):50-56,64.(in Chinese)
[89]
LI Y Y,TANG D,DU Y L,et al.Fatty liver mediated by peroxisome proliferator-activated receptor-α DNA methylation can be reversed by a methylation inhibitor and curcumin[J].Journal of Digestive Diseases,2018,19(7):421-430.
[62]
李晓玲,于光辉,崔小荣,等.猪A-FABP基因真核表达载体的构建及其组织表达[J].中国畜牧杂志,2018,54(1):103-107. LI X L,YU G H,CUI X R,et al.Constructing of eukaryotic expressing vector and tissues expression of A-FABP gene in pig[J].Chinese Journal of Animal Science,2018,54(1):103-107.(in Chinese)
[64]
ZENG Z,YU B,MAO X B,et al.Effects of dietary digestible energy concentration on growth,meat quality,and PPARγ gene expression in muscle and adipose tissues of Rongchang piglets[J].Meat Science,2012,90(1):66-70.
[66]
边连全,许云贺,苏玉虹,等.能量水平对猪H-FABP基因表达量及对肉质的影响[C]//中国畜牧兽医学会养猪学分会第五次全国会员代表大会暨养猪业创新发展论坛论文集.桂林:中国畜牧兽医学会,2010:329-333. LIAN B Q,XU Y H,SU Y H,et al.Effects of energy level on pig H-FABP gene expression and meat quality[C]//Proceedings of the 5th National Member Congress and Innovation and Development Forum of Pig Industry of Pig Science Branch of China Association of Animal Science and Veterinary Medicine.Guilin:Chinese Association of Animal Science and Veterinary Medicine,2010:329-333.(in Chinese)
[68]
LI Y H,LI F N,DUAN Y H,et al.Low-protein diet improves meat quality of growing and finishing pigs through changing lipid metabolism,fiber characteristics,and free amino acid profile of the muscle[J].Journal of Animal Science,2018,96(8):3221-3232.
[70]
张克英,陈代文,罗献梅,等.饲粮理想蛋白水平对猪肉品质的影响[J].四川农业大学学报,2002,10(1):12-16. ZHANG K Y,CHEN D W,LUO X M,et al.Effects of dietary levels of ideal protein on pig meat quality[J].Journal of Sichuan Agricultural University,2002,10(1):12-16.(in Chinese)
[72]
KNOWLES T A,SOUTHERN L L,BIDNER T D,et al.Effect of dietary fiber or fat in low-crude protein,crystalline amino acid-supplemented diets for finishing pigs[J].Journal of Animal Science,1998,76(11):2818-2832.
[74]
ZOU T D,MAO X B,YU B,et al.Effects of dietary energy density and apparent ileal digestible lysine:digestible energy ratio on growth performance,meat quality,and peroxisome proliferator-activated receptor gamma (PPARγ) gene expression of muscle and adipose tissues in Landrace×Rongchang crossbred pigs[J].Livestock Science,2014,167:219-226.
[76]
MA X Y,LIN Y C,JIANG Z Y,et al.Dietary arginine supplementation enhances antioxidative capacity and improves meat quality of finishing pigs[J].Amino Acids,2010,38(1):95-102.
[78]
胡诚军,张婷,李华伟,等.饲粮添加亮氨酸和谷氨酸对肥育猪生长性能、胴体性状和肉品质的影响[J].动物营养学报,2017,29(2):590-596. HU C J,ZHANG T,LI H W,et al.Effects of dietary leucine and glutamate on growth performance,carcass traits and meat quality of finishing pigs[J].Chinese Journal of Animal Nutrition,2017,29(2):590-596.(in Chinese)
[80]
ZHANG H B,DONG X W,WANG Z S,et al.Dietary conjugated linoleic acids increase intramuscular fat deposition and decrease subcutaneous fat deposition in Yellow Breed×Simmental cattle[J].Animal Science Journal,2016,87(4):517-524.
[82]
MOREL P C H,JANZ J A M,ZOU M,et al.The influence of diets supplemented with conjugated linoleic acid,selenium,and vitamin E,with or without animal protein,on the composition of pork from female pigs[J].Journal of Animal Science,2008,86(5):1145-1155.
[88]
GRACIA A,ELCOROARISTIZABAL X,FERNÁNDEZ-QUINTELA A,et al.Fatty acid synthase methylation levels in adipose tissue:effects of an obesogenic diet and phenol compounds[J].Genes & Nutrition,2014,9(4):411.
