Effects of Adding Pectic Oligosaccharide into Oxidized Diet on Antioxidant Capacity and Histomorphology of Jejunum and Liver in Weaned Rats
WANG Xu1,2, MAO Xiangbing1, SUN Rui1, FAN Xiangqi1, YU Wei1, DOU Yisong1
1. Key Laboratory for Animal Disease-Resistance Nutrition of Ministry of Education, Key Laboratory of Animal Disease-Resistant Nutrition and Feed of Ministry of Agriculture and Rural Affairs, Key Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;
2. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China
Abstract：This experiment was conducted to determine whether pectic oligosaccharide (POS) administration could alleviate the negative effect of oxidized diet on antioxidant capacity and histomorphology in jejunum and liver of weaned rats. Eighteen Wistar rats weaned at 21 days of age (approximate average weight was 47.32 g) were allotted into 3 groups (n=6) fed by normal diet, oxidized diet and oxidized diet with 200 mg/kg POS for 21 d. The results showed that acid value and peroxide value in oxidized diet were increased while POS administration, to some extent, decreased acid value and peroxide value in oxidized diet, and POS had high 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical scavenging ability. Compared with normal diet, feeding oxidized diet significantly decreased the body weight gain and feed intake (P<0.05), significantly decreased the total superoxide dismutase and glutathione peroxidase activities and total antioxidant capacity of jejunum and liver (P<0.05), significantly decreased the malondialdehyde content of jejunum and liver (P<0.05), and lead to the histomorphology damage of jejunum and liver in weaned rats. Compared with oxidized diet, adding POS into the oxidized diet, to some extent, significantly improved the negative effect of oxidized diet on body weight gain and feed intake (P<0.05), significantly increased the total antioxidant capacity and total superoxide dismutase activity of jejunum and liver (P<0.05), significantly decreased the malondialdehyde content of jejunum and liver (P<0.05), and relieved the histomorphology damage of jejunum and liver in weaned rats. These results suggest that adding pectic oligosaccharide into the oxidized diet can attenuate the oxidized diet-induced decrease of growth performance and damage of jejunum and liver histomorphology in weaned rats, which can be associated with the improvement of antioxidant capacity stimulated by POS.
王旭, 毛湘冰, 孙锐, 范祥奇, 余伟, 豆屹松. 氧化饲粮中添加果胶寡糖对断奶大鼠空肠与肝脏组织形态和抗氧化能力的影响[J]. 动物营养学报, 2021, 33(3): 1716-1723.
WANG Xu, MAO Xiangbing, SUN Rui, FAN Xiangqi, YU Wei, DOU Yisong. Effects of Adding Pectic Oligosaccharide into Oxidized Diet on Antioxidant Capacity and Histomorphology of Jejunum and Liver in Weaned Rats. Chinese Journal of Animal Nutrition, 2021, 33(3): 1716-1723.
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