为了探讨半胱胺和大豆黄酮对东北细毛羊肝脏、肌肉和皮肤组织中IGF-Ⅰ mRNA表达量的影响，选取体重27～28 kg，5～6月龄健康的东北细毛羊育成羊21只，随机分成7组，每组3个重复，每个重复1只，采用实时荧光定量PCR法检测肝脏、肌肉和皮肤组织中IGF-Ⅰ mRNA相对表达量。结果表明：(1)半胱胺和大豆黄酮均能显著提高东北细毛羊的生长性能(P<0.05)，其中以7.5 mg/kg BW半胱胺组和7.5 mg/kg BW半胱胺+3 mg/kg BW 大豆黄酮组效果较佳；(2)半胱胺和大豆黄酮对东北细毛羊肝脏和肌肉中IGF-Ⅰ mRNA表达量有显著的提高作用(P<0.05)，但各试验组之间差异不显著(P>0.05)；(3)对皮肤中表达量的影响：对照组与添加3和6 mg/kg BW大豆黄酮组和7.5 mg/kg BW半胱胺+3 mg/kg BW大豆黄酮组之间差异显著(P<0.05)，与添加7.5和15 mg/kg BW半胱胺组和15 mg/kg BW半胱胺+6 mg/kg BW大豆黄酮组之间差异不显著(P>0.05)。由结果可知，半胱胺和大豆黄酮均能提高肝脏和肌肉中IGF-Ⅰ mRNA的表达量，但协同应用并没有促使IGF-Ⅰ mRNA表达量在肝脏、肌肉和皮肤组织中的明显提高，综合分析以添加3 mg/kg BW大豆黄酮效果最佳。

In order to explore the effects of cysteamine and daidzein on gene expression of IGF-Ⅰ mRNA in the tissue of liver, muscle and skin in Northeast fine-wool sheep. Twenty-one healthy Northeast fine-wool sheep with body weight of 27～28 kg and age of 5～6-month-old were randomly divided into 7 groups with 3 replicates and each replicate had 1 sheep. Using real-time fluorescence quantitative PCR detected the IGF-Ⅰ mRNA expression on liver, muscle and skin. The results showed as follows: 1) both cysteamine and daidzein could significantly enhance the growth performance of Northeast fine-wool sheep (P<0.05), and the 7.5 mg/kg BW cysteamine group and 7.5 mg/kg BW cysteamine＋3 mg/kg BW daidzein group had the better effects; 2) cysteamine and daidzein could evidently increased the expression of gene IGF-Ⅰ in the liver and muscle of Northeast fine-wool sheep (P<0.05), but no significant difference was found among trial groups (P>0.05); 3) compared with the control group, the IGF-Ⅰ mRNA expression on the skin in 3 mg/kg BW daidzein group, 6 mg/kg BW daidzein group and 7.5 mg/kg BW cysteamine＋3 mg/kg BW daidzein group were significantly improved (P<0.05), but no significant difference was found among the control group, 7.5 mg/kg BW cysteamine group, 15 mg/kg BW cysteamine group and 15 mg/kg BW cysteamine＋6 mg/kg BW daidzein group (P>0.05). It was concluded that cysteamine and daidzein could improve the IGF-Ⅰ mRNA expression in liver and muscles, but cooperation application did not make the gene IGF-Ⅰ mRNA expression obviously improved in the liver, muscle and skin tissue. In summary, adding 3 mg/kg BW of daidzein had the best effect.［Chinese Journal of Animal Nutrition, 2009，21(6):967-973］