The study was conducted to investigate the effects of dietary ideal protein level on tenderness and mRNA expression level of CaN-NFAT signaling pathway related to0020proteins and CAST of longissimus dorsi in finishing pigs. Ninety crossbred pigs (Duroc × Landrace × Large white, about 50 kg BW) were randomly allocated into 3 treatments with 3 replicates and per treatment 10 heads per replicate. Pigs in the three treatments were fed diets with the same energy level and ideal protein levels at 12%, 16% and 20%, respectively. All pigs were slaughtered after 58 days experiment and muscle samples were collected for the analysis of shear force, as well as mRNA expression levels of CAST, CaN, CaM and NFAT of longissimus dorsi in finishing pigs were determined by real-time PCR. The results showed as follows: 1) High dietary protein level significantly increased the shear force (P<0.05) and mRNA expression levels of CaN and CAST of longissimus dorsi (P<0.01). 2) The shear force was positively correlated with the mRNA expression levels of CAST (coefficient correlation=0.713, P<0.01); the mRNA expression levels of NFAT (coefficient correlation=0.536, P<0.05) and CaM (coefficient correlation=0.546, P<0.05) were positively correlated with that of CaN; the mRNA expression levels of CaM and NFAT were positively correlated (coefficient correlation=0.623, P<0.05) to each other. The results indicate that high dietary protein level can significantly decrease the tenderness, but increase mRNA expression levels of CAST and CaN of longissimus dorsi; the tenderness of longissimus dorsi is positively correlated with CAST mRNA expression level, but is not correlated with the CaN-NFAT signaling pathway.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1153 -1160］
［1］KAO S C, WU H, XIE J M, et al. Calcineurin/NFAT signaling is required for neuregulin-regulated schwann cell differentiation[J]. Science, 2009, 323(5914):651-654.
[2]KOOHMARAIE M．Biochemical factors regulation the toughening and tenderization process of meat[J]. Meat Science, 1996, 43:S193．
[3]MARTINS P A, SALIC K, GLADKA M M, et al. MicroRNA-199b targets the nuclear kinase Dyrk1a in an auto-amplification loop promoting calcineurin/NFAT signaling[J]. Nature Cell Biology, 2010, 12:1220-1227.
[4]ABDUL H M, SAMA M A, FURMAN J L, et al. Cognitive decline in Alzheimer’s disease is associated with selective changes in calcineurin/NFAT signaling[J]. The Journal of Neuroscience, 2009, 29(41):12957-12969.
[5]COURTWRIGHT A, REIHANI S S, ARBISER J L, et al. Secreted frizzle-related protein 2 stimulates angiogeneses via a calcineurin/NFAT signaling pathway[J]. Current Issue, 2011, 71(1):471-484.
[6]DONNELL S M, THOMAS W, KUBO H, et al. CaMKⅡ negatively regulates calcineurin/NFAT signaling in cardiac myocytes[J]. Circulation Research, 2009, 105(4):316-325.
[7]HOUSER S R, MOLKENTIN J D. Does contractile Ca2+ control calcineurin/NFAT signaling and pathological hypertrophy in cardiac myocytes[J]. Science Signaling, 2008,25 (1):1126-1130.
[8]PALMER B R, ROBERTS N, HICKFORD J G H, et al. Rapid communication: PCR-RFLP for MspⅠ and NcoⅠ in the ovine calpastatin gene[J]. Journal of Animal Science, 1998, 76:1499-1450.
[9]CIOBANU D C, BASTIAANSEN J W M, LONERGAN S M, et al. New alleles in calpastatin gene are associated with meat quality traits in pigs[J]. Journal of Animal Science, 2004, 82:2829-2838.
[10]KURYL J, KAPELANSKI W, PIERZCHALA M. Preliminary observations on the effect of calpastatin gene (CAST) polymorphism on carcass traits in pigs[J]. Animal Science Papers and Reports, 2003, 21:87-95.
[11]COCKETT N E, SHAY T L, GREEN R D, et al. Rapid communication: a TaqⅠrestriction fragment length polymorphism in the bovine calpastatin gene[J]. Journal of Animal Science, 1995, 73(12):3790.
[12]BUCHHOLZ M, SCHATZ A, WAGNER M, et al. Overexpression of c-myc in pancreatic cancer caused by ectopic activation of NFATc1 and the Ca2+/calcineurin signaling pathway[J]. European Molecular Biology Organization, 2006, 25:3714-3724.
[13]张勇,高彦,朱宇旌,等.不同饲喂方式对猪背最长肌钙蛋白酶抑制蛋白和钙蛋白酶基因表达及剪切力的影响[J].动物营养学报，2010，22(3):640-646.
[14]张勇,李方方,朱宇旌,等.日粮不同蛋白质水平对猪骨骼肌钙蛋白酶抑制蛋白和钙蛋白酶基因表达及嫩度的影响[J].动物营养学报,2008,20(3):360-365.
[15]LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2－△△Ct method[J]. Method, 2001, 25(4):402-408.
[16]翟峰,张勇.钙蛋白酶抑制蛋白与肌肉嫩度相关性[J].中国饲料,2007，16:11-13.
[17]GOERL K F, EILERT S J, MANDIGO R W, et al. Pork characteristics as affected by two populations of swine and six crude protein levels[J]. Journal of Animal Science, 1995, 73:3621-3626.
[18]DAVEY R J. Growth and carcass characteristics of high-and low-fat swine fed diets varying in protein and lysine content[J]. Journal of Animal Science, 1976, 43:598-605．
[19]张克英,陈代文,罗献梅.饲粮理想蛋白水平对猪肉品质的影响[J].四川农业大学学报,2002(1):37-39.
[20]THOMSON B C, HOSKING B J, SAINZ R D, et al. The effect of nutrional status on protein degradation and components of the calpain system in skeletal muscle of weaned wether lambs[J]. The Journal of Agricultural Science, 1997, 129:471-477.
[21]HEMAN E E, HUFF-LONERGAN E, DAVENPORT G M, et al. Effcet of dietary protein on calpastatin in CaNine skeletal muscle[J]. Journal of Animal Science, 2003, 81:2199-2205.
[22]PARR T, JEWELL K K, SENSKY P L, et al. Expression of calpastatin isoforms in muscle and functionality of multiple calpastatin promoters[J]. Archives of Biochemistry and Biophysics, 2004, 427:8-15.
[23]HIAN M A, MORTON J D, KENT M P. Intermuscular variation in tenderness：association with the ubiquitous and muscle-specific calpains[J]. Journal of Animal Science, 2001, 79(1):122-l32.
[24]李德发.营养调控肉品质量的研究现状及发展趋势[M]//动物营养研究进展.北京:农业科技出版社,2004:7-14.
[25]KOOHMARAIE M, GEESINK G H. Contribution of postmortem muscle biochemistry to the delivery of consistent meat quality with particular focus on the calpain system[J]. Meat Science, 2006 (74): 34-43.
[26]孙立彬,孟和,王起山,等.猪2型钙蛋白酶抑制蛋白基因cDNA的克隆序列分析[J].上海交通大学学报:农业科学版,2005,23(3):66-69.
[27]武艳群,吴旧生,赵晓枫,等.猪CAST基因与肌纤维组织学特性及屠宰性状的相关性分析[J].遗传,2007,29(1):65-69.
[28]KLONT R E, EIKELNBOOM L B. Muscle fibre type and meat quality[J]. Meat Science, 1998, 49(Suppl.1):S219-S229.
[29]KARLSSON A H , KLONT R E, FERNANDEZ X. Skeletal muscle fibres as factors for pork quality[J]. Livestock Production Science, 1999, 60:255-269.
[30]CHANG K C, DA COST N, BLACKLEY R, et al. Relationships of myosin heavy chain fibre types to meat quality traits in traditional and modern pigs[J]. Meat Science, 2003, 64:93-103.
[31]PARSONS S A, MILLAY D P, WILKINS B J, et al. Genetic loss of calcineurin blocks mechanical overload-induced skeletal muscle fiber type switching but not hypertrophy[J]. Journal of Biological Chemistry, 2004, 279(25):26192-26200.
[32]TIMMERMAN L A, CLIPSTONE N A, HO S N, et al. Rapid shuttling of NFAT in discrimination of Ca2+ signals and immunosuppression[J]. Nature, 1996, 383(6603):837-840.
[33]CHAKKALAKAL J V, HARRISON M A, CARBONETTO S, et al. Stimulation of calcineurin signaling attenuates the dystrophic pathology in mdx mice[J]. Human Molecular Genetics, 2004, 13(4):379-388.
[34]SAKUMA K, NISHIKAWA J, NAKAO R, et al. Calcineurin is a potent regulator for skeletal muscle regeneration by association with NFATc1 and GATA-2[J]. Acta Neuropathologica, 2003, 105(3):271-280.
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