A full-length cDNA coding glucokinase (GK) was cloned from Pengze crucian carp (Carassius auratus var. Pengze) by RT-PCR and rapid amplification of cDNA ends (RACE) methods. The cDNA sequence obtained is of 2 050 bp length with a 1 431 bp open reading frame (ORF) encoding 476 amino acids. The GK protein has a calculated molecular weight of 53.78 ku and isolectric point of 5.14. The main domains of GK, such as ATP-binding domain, glucose-binding amino acids, hexokinases signature, N-linked glycosylation sites, cell attachment sequence and glycosaminoglycan attachment site for the Pengze crucian carp are basically conservative compared with other vertebrates. The amino acid sequence has a high similarity to GK of other species, and the percent identity compared with common carp, human and rat are 98.1%, 79.8% and 79.1%, respectively. Tissue distribution of GK mRNA in brain, white muscle, spleen, mesenteric adipose tissue and liver of Pengze crucian carp was analyzed by semi-quantitative RT-PCR method using β-actin gene as internal control. The result showed that the expression level of GK mRNA in liver was significantly higher than that in spleen, mesenteric adipose tissue and brain (P<0.05). GK mRNA did not be detected in white muscle.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1167 -1175］
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