本文旨在研究不同加工方法对白酒糟营养价值和体外瘤胃发酵特性的影响。以未处理白酒糟作为对照(CK组)，按白酒糟的加工方式不同设4个试验组：黄孢原毛平革菌发酵白酒糟(发酵，F组)、黄孢原毛平革菌发酵粉碎白酒糟(粉碎+发酵，CF组)、黄孢原毛平革菌发酵氨化白酒糟(氨化+发酵，AF组)、黄孢原毛平革菌发酵粉碎、氨化白酒糟(粉碎+氨化+发酵，CAF组)。测定各组白酒糟常规营养成分及体外瘤胃发酵指标。结果表明：1）黄孢原毛平革菌发酵能极显著降低白酒糟酸性洗涤木质素（ADL）含量、极显著提高粗蛋白质（CP）含量(P<0.01)，粉碎+氨化+发酵3种加工方式联合处理效果最好。2）与CK组相比，CF组、AF组和CAF组理论最大产气量和产气速率极显著提高、产气延滞时间极显著降低(P<0.01)；CAF组产气量、理论最大产气量和产气速率极显著高于其他各试验组(P<0.01)。3）CAF组菌体蛋白（MCP）含量显著高于其他各组(P<0.05)；AF组和CAF组氨态氮（NH3-N）浓度（P<0.05)、总挥发性脂肪酸(TVFA)浓度(P<0.05)、丙酸比例(P<0.01)显著或极显著高于其他各组，乙酸/丙酸极显著低于其他各组(P<0.01)。结果提示：对白酒糟进行粉碎、氨化处理，可促进黄孢原毛平革菌对白酒糟木质素的降解，进而提高其营养价值、改善瘤胃发酵，粉碎、氨化、发酵复合加工效果最好。
his experiment was conducted to study the effects of different processing methods on nutritive value and in vitro ruminal fermentation characteristics of distilled grain (DG). The unprocessed DG was used as the control (CK group), and 4 experimental groups were set by the different processing methods, which were DG fermented with P. chrysosporium (ferment, F group), crushed DG fermented with P. chrysosporium (crush+ferment, CF group), ammoniated DG fermented with P. chrysosporium (ammoniate+ferment, AF group), as well as crushed and ammoniated DG fermented with P. chrysosporium (crush+ammoniate+ferment, CAF group). The normal nutrient composition and in vitro ruminal fermentation indices of DG were determined. The results showed as follows: 1) the acid detergent lignin (ADL) and crude protein (CP) contents were significantly decreased and increased by the fermentation of P. chrysosporium, respectively (P<0.01), and the combined treatment of crush, amination and fermentation had the best effect. 2) Compared with CK group, the theoretical maximum gas production and gas production speed in CF, AF and CAF group were all significantly increased (P<0.01), as well as the gas lag time in those groups (P<0.01); the gas production, theoretical maximum gas production and gas production speed in CAF group were significantly higher than those in the other experimental groups (P<0.01). 3) The microprotein (MCP) content in CAF group was significantly higher than that in the other groups (P<0.05); the NH3-N concentration (P<0.05) and total volatile fatty acid (TVFA) concentration (P<0.05) and proportion of propionate (P<0.01) in AF and CAF group were significantly higher than that in the other groups, but the ratio of acetate and propionate was significantly lower than that in the other groups (P<0.01). The results indicate that the process of crush and amination facilitates the degradation of lignin in DG by P. Chrysosporium, which in turn upgrades the nutritive value of DG and improves ruminal fermentation. The combined treatment of and crush, amination and fermentation is the most efficient.［Chinese Journal of Animal Nutrition, 2011, 23（8）：1422 -1429］
［1］李政一,周定,侯文华.酒糟资源化研究[J].环境科学学报,2000,(20)9:145-149.
[2]ANDERSSON B, HENRYSSON T. Accumulation and degradation of dead-end metabolites during treatment of soil contaminated with polycyclic aromatic hydrocarbons with five strains of white-rot fungi[J]. Applied Microbiology and Biotechnology, 1996, 46:647-652.
[3]SCHEEL T, HOFER M, LUDWIG S, et al. Differential expresstion of manganess peroxidase and laccase in white-rot fungi in the presence of manganese or aromatic compound[J]. Applied Microbiology and Biotechnology, 2000, 54:686-691.
[4]农业部全国饲料工作办公室.饲料工业标准汇编[M].北京:中国标准出版社,2002.
[5]MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research and Development, 1988, 28:7-55.
[6]VAN SOEST P J, ROBERTSON J B, LEWIS B A. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition[J]. Journal of Dairy Science, 1991, 74(10):3583-3597.
[7]冯仰廉,王加启,杨红建,等.中华人民共和国农业行业标准NY/T 815—2004[S].北京:中华人民共和国农业部出版,2004:4-21.
[8]MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research and Development, 1988, 28:7-55.
[9]冯宗慈.通过比色测定瘤胃液氨氮含量方法的改进[J].内蒙古畜牧科学,1993,4:40-41.
[10]MAKKAR H P S, BECKER K. Purine quantification in digesta form ruminants by spectrophotometic and HPLC methods[J]. British Nutrition, 1999, 81:107-112.
[11]SCHOFIELD P, PITT R E, PELL A N. Kinetics of fiber digestion from in vitro gas production[J]. Journal of Animal Science, 1994, 72:2980-2991.
[12]陈谊,康鸿明,杭怡琼,等.白腐真菌降解稻草喂猪试验[J].上海农业学报,2004,20(3):109-110.
[13]王立志,赵碧刚,王之盛.黄孢原毛平革菌降解白酒糟中木质素的影响因素[J].四川农业大学学报,2009,27(2):203-207.
[14]HUANG D L, ZENG G M. Biotransformation of rice straw by Phanerachaete chrysosporium and the related ligninolytic enzymes[J]. International Journal of Biotechnology, 2008, 10(1):86-92.
[15]NIEOLE M, CHMABERLNAD H, GEIGER J P, et al. Detemrination of the respiration kineticsfor mycelial pellets of Phanerochaete chrysosporium[J]. Applied Environmental Microbiology, 1992, 58(5):1727-1739.
[16]SERBONIIK E, MESSNER K, FOISNER R. Ultrastructural localization of Phanerochaete chrysosporium by immunogold labeling[J]. Current Microbiology, 1988(16):221-227.
[17]NSAHLAI I V, SIAW D E K A, OSUJI P O. The relationships between gas production and chemical composition of 23 browses of the genus Sesbania[J]. Journal Science of Food and Agriculture, 1994, 65:13-20.
[18]AKIN D E, BENNER R. Degradation of polysaccharides and lignin by ruminal bacteria and fungi[J]. Applied and Environmental Microbiology, 1988, 54:1117-1125.
[19]杨红建，冯仰廉.不同纤维素与淀粉比率等氮纯化日粮瘤胃发酵挥发性脂肪酸产生量[J].中国畜牧杂志,2003,39(5):9-11.
[20]胡红莲,卢德勋,刘大程,等.日粮不同NFC/NDF比对奶山羊瘤胃pH、挥发性脂肪酸及乳酸含量的影响[J].动物营养学报,2010,22(3):595-601.
[21]余群莲,王之盛,薛白,等.饲粮中结构性碳水化合物与非结构性碳水化合物比例对降解后白酒糟体外发酵特性的影响[J].动物营养学报,2010,22(4):956-963.
[22]陈喜斌，冯仰廉.瘤胃VFA产量与瘤胃可发酵有机物关系的研究[J].动物营养学报，1996，8（2）：32-36.
[23]谭支良,卢德勋,胡明,等.绵羊日粮中不同碳水化合物比例对瘤胃内环境参数的影响[J].动物营养学报,2000,12:42-47.
[24]RUSSELL J B, CONNOR J D O, FOX D G, et al. A net carbohydrate and protein system for evaluating cattle diets: ruminal fermentation[J]. Journal of Animal Science, 1992, 70:3551-3561.
[25]NOCEK J E, RUSSELL J B. Protein and energy as an integrated system. Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production[J]. Journal of Dairy Science, 1988, 71:2070-2107.
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