本试验旨在研究精氨酸-甘氨酸-谷氨酰胺（Arg-Gly-Gln）对断奶仔猪空肠体外酶活及细胞增殖与凋亡的影响。试验采用单因子设计，设6个处理，培养液分别添加Arg-Gly-Gln 0、0.28、0.56、1.11、2.23、4.45 mmol/L，每个处理8个重复，每个重复1个空肠组织块。检测培养液中乳酸脱氢酶（LDH）活力、肠组织中谷氨酰胺酶和二胺氧化酶（DAO）活力以及5-溴脱氧尿嘧啶核苷（BrDU）、半胱氨酸的天冬氨酸特异性蛋白水解酶-3(Caspase-3)阳性细胞百分比的变化。结果表明：1）与无添加的培养液相比，除添加0.56、2.23 mmol/L Arg-Gly-Gln在6 h时以及添加1.11 mmol/L Arg-Gly-Gln在24 h时无显著差异(P>0.05)外，其余各时间点、各添加Arg-Gly-Gln的培养液中LDH活力均显著（P<0.05）或极显著（P<0.01）降低；2）与无添加的培养液相比，培养液添加1.11、2.23、4.45 mmol/L Arg-Gly-Gln空肠组织谷氨酰胺酶活力分别提高了27.69%、46.15%、83.08%(P<0.01)，培养液添加2.23、4.45 mmol/L Arg-Gly-Gln空肠组织DAO活力分别提高了66.34%和110.89%(P<0.01)；3）随着培养液Arg-Gly-Gln水平的提高，BrdU阳性细胞百分比增加，Caspase-3阳性细胞百分比降低。结果提示：Arg-Gly-Gln能够缓解细胞损伤并降低细胞膜通透性，提高空肠组织谷氨酰胺酶和DAO的活力，增强肠细胞对Gln的利用；Arg-Gly-Gln能够促进断奶仔猪离体空肠细胞增殖，抑制由Caspase-3介导的细胞凋亡，从而提高肠道黏膜的屏障功能。
This trial was conducted to study the effect of Arg-Gly-Gln on jejunum enzyme activities, cell proliferation and apoptosis of weaned piglets in vitro. A single-factor design was adopted and six treatments were set, which were supplementation of Arg-Gly-Gln at 0, 0.28, 0.56, 1.11, 2.23 and 4.45 mmol/L in culture medium, respectively. There were eight replicates in each treatment and one jejunum tissue block in each replicate. The activity of lactate dehydrogenase（LDH） in culture medium, activities of glutaminase and diamine oxidase （DAO） in intestinal tissues, percentages of BrdU-positive cells and Caspase-3-positive cells were determined. The results showed as follows: 1) compared with that in culture medium without supplemental Arg-Gly-Gln, LDH activity in culture medium supplemented with Arg-Gly-Gln at different time point significantly decreased （P<0.05 or P<0.01）, excluding the culture medium supplemented with Arg-Gly-Gln at 0.56, 2.23 mmol/L at 6 h (P>0.05) and 1.11 mmol/L at 24 h (P>0.05); 2) compared with that in culture medium without supplemental Arg-Gly-Gln, glutaminase activity of jejunum tissue in culture medium supplemented with Arg-Gly-Gln at 1.11, 2.23 and 4.45 mmol/L increased by 27.69%, 46.15% and 83.08% (P<0.01), respectively, and DAO activity of jejunum tissue in culture medium supplemented with Arg-Gly-Gln at 2.23 and 4.45 mmol/L increased by 66.34% and 110.89% (P<0.01), respectively; 3) with the increasing of Arg-Gly-Gln levels in culture medium, percentage of BrdU-positive cells increased, but that of caspase-3-positive cells reduced. The results indicate that Arg-Gly-Gln can not only alleviate cell damage and reduce cell membrane permeability but also enhance the glutaminase and DAO activities, and promote the utilization of glutamine by intestinal cells. In addition, Arg-Gly-Gln can promote the cell proliferation of jejunum and inhibit apoptosis mediated by Caspase-3, resulting in the enhancement of barricade function of intestinal mucous membrane.［Chinese Journal of Animal Nutrition, 2011, 23（9）：1475 -1482］
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