本研究旨在探讨在饲粮中添加猪源罗伊氏乳酸杆菌（Lactobacillus reuteri）对断奶仔猪生长性能和血清指标的影响。选用平均体重为（16.57±0.23） kg的断奶仔猪（杜洛克×长白×大白三元杂交）64头，随机分成4组，每组4个重复，每个重复4头。对照组饲喂基础饲粮，试验组饲粮分别在基础饲粮中添加0.25%、0.50%、0.75%的猪源罗伊氏乳酸杆菌，试验期为30 d。结果表明：1）添加0.50%和0.75%猪源罗伊氏乳酸杆菌组断奶仔猪平均日增重分别比对照组提高了7.56%（P<0.05）和20.07%（P<0.05），料重比分别比对照组降低了1.96%（P>0.05）和14.90%（P<0.05）；2）饲粮中添加猪源罗伊氏乳酸杆菌能够极显著降低断奶仔猪血清中白蛋白与球蛋白比值（P<0.01），并极显著增加血清中干扰素γ含量（P<0.01）；3）添加0.50%和0.75%猪源罗伊氏乳酸杆菌组断奶仔猪血清中结合珠蛋白含量分别比对照组降低了8.79%（P<0.05）和9.34%（P<0.05）。结果提示，饲粮中添加猪源罗伊氏乳酸杆菌能够提高断奶仔猪机体免疫能力，而当其达到一定添加剂量时，对于提高断奶仔猪生长性能的效果表现明显。
The objective of this study was to evaluate the effects of supplementation of freeze-dried Lactobacillus reuteri on growth performance and serum indices of weaned piglets. Sixty-four crossbred (Duroc×Landrace×Large White) weaned piglets with averge body weight of (16.57±0.23) kg were selected and randomly distributed into four groups with four replicates per group and four pigs per replicate comprising of control (basal diet), and 0.25%, 0.50%, and 0.75% freeze-dried Lactobacillus reuteri supplementation groups. The experiment lasted for 30 d. The results showed that: 1) compared with the control group, the average daily gain in 0.50% and 0.75% Lactobacillus reuteri groups were significantly increased by 7.56% (P<0.05) and 20.07% (P<0.05), respectively, and the feed/gain were decreased by 1.96% (P>0.05) and 14.90% (P<0.05), respectively; 2) the Lactobacillus reuteri supplementation could significantly decrease the albumin/globulin (P<0.01) in serum compared with the control group, and also significantly increase the serum IFN-γ content (P<0.01); 3) compared with the control group, the haptoglobin contents in 0.50% and 0.75% Lactobacillus reuteri groups were significantly decreased by 8.79% (P<0.05) and 9.34% (P<0.05), respectively. In conclusion, Lactobacillus reuteri supplementation could improve the immune ability of weaned piglets, and the obvious promotion on growth performance could obtained in adequate amounts of supplementaion.［Chinese Journal of Animal Nutrition, 2011, 23（9）：1553 -1559］
［1］NG S C, HART A L, KAMM M A, et al. Mechanisms of action of probiotics: recent advances[J]. Inflammation Bowel Disease, 2009, 15(2):300-310.
[2]CASAS I A, DOBROGOSZ W J. Validation of the probiotic concept: Lactobacillus reuteri confers broad-spectrum protection against disease in humans and animals[J]. Microbial Ecology in Health and Disease, 2000, 12:247-285.
[3]CLEUSIX V, LACROIX C, VOLLENWEIDER S, et al. Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria[J]. BMC Microbiology, 2007, 7(101):1-9.
[4]TALARICO T L, CASAS I A, CHUNG T C, et al. Production and isolation of reuterin, a growth inhibitor produced by Lactobacillus reuteri[J]. Antimicrobial Agents Chemotherapy, 1988, 32:1854-1858.
[5]MITSUOKA T. The human gastrointestinal tract[M]//WOOD B J B. The lactic acid bacteria in health and disease. London: Elsevier, 1992:69-114.
[6]BON M L, DAVIES H E, GLYNN C, et al. Influence of probiotics on gut health in the weaned pig[J]. Livestock Science, 2010, 133(1):179-181.
[7]CROSS M L. Microbes versus microbes: immune signals generated by probiotic lactobacilli and their role in protection against microbial pathogens[J]. FEMS Immunology Medicine Microbiology, 2002, 34(4):245-253.
[8]SIEO C C, ABDULLAH N, TAN W S, et al. Influence of β-glucanase producing Lactobacillus strains on intestinal characteristics and feed passage rate of broiler chickens[J]. Poultry Science, 2005, 84(5):734-741.
[9]LEE Y K, LIM C Y, TENG W L, et al. Quantitative approach in the study of adhesion of lactic acid bacteria to intestinal cells and their competition with enterobacteria[J]. Application Environment Microbiology, 2000, 66(9):3692-3697.
[10]GUERRA N P, BERNARDEZ P F, MENDEZ J, et al. Production of four potentially probiotic lactic acid bacteria and their evaluation as feed additives for weaned piglets[J]. Animal Feed Science and Technology, 2007, 134(1/2):89-107.
[11]NOUSIAINEN J, SETL J. Lactic acid bacteria as animal probiotics[M]//SALMINEN S, VON WRIGHT A. Lactic acid bacteria. New York: Marcel Dekker Inc., 1993:315-356.
[12]张永勇.复合益生素对断奶仔猪生长性能和血液生化相关指标的影响[D].硕士学位论文.长沙：湖南农业大学,2007:26-27.
[13]FUJIWARA D, INOUE S, WAKABAYASHI H, et al. The anti-allergic effects of lactic acid bacteria are strain dependent and mediated by effects on both Th1/Th2 cytokine expression and balance[J]. International Archives of Allergy and Immunology. 2004, 135(3):205-215.
[14]OUWEHAND A, ISOLAURI E, SALMINEN S, et al. The role of the intestinal microflora for the developmen of the immune system in early childhood[J]. European Journal of Nutrition, 2002, 41(1):32-37.
[15]SEGAWA S, NAKAKIA Y, TAKATA Y, et al. Effect of oral administration of heat-killed Lactobacillus brevis SBC8803 on total and ovalbumin-specific immunoglobulin E production through the improvement of Th1/Th2 balance[J]. International Journal of Food Microbiology, 2008, 121(1):1-10.
[16]MOHAMADZADEH M, OLSON S, KALINA W V, et al. Lactobacilli activate human dendritic cells that skew T cells toward T helper 1 polarization[J]. Proceedings of the National Academy of Sciences of the United States of America, 2005, 102(8):1-5.
[17]PETERSEN H H, NIELSEN J P, HEEGAARD P M. Application of acute phase protein measurements in veterinary clinical chemistry[J]. Veterinarian Research, 2004, 35(2):163-187.
[18]GRUYS E, TOUSSAINT M J, UPRAGARIN N, et al. Acute phase reactants, challenge in the near future of animal production and veterinary medicine[J]. Journal of Zhejiang University Science, 2005, 6B(10):941-947.
[19]SORENSEN N S, TEGTMEIER C, ANDRESEN L O, et al. The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis[J]. Veterinary Immunology and Immunopathology, 2006, 113(1/2):157-168.
[20]FRANCISCO C J, BANE D P, WEIGEL R M, et al. The influence of pen density, weaning age, and feeder space on serum haptoglobin concentration in young growing swine[J]. Journal of Swine Health and Production, 1996, 4(2):67-71.
[21]LIPPERHEIDE C, DIEPERS N, LAMPREAVE F, et al. Nephelometic determination of haptoglobin plasma concentrations in fattening pigs[J]. Journal of Veterinary Medicine, 1998, 45(9):543-550.
[22]DICKHFER D. Haptoglobin as a screening parameter for respiratory diseases of swine [D]. Ph.D. thesis. Hannover: University of Veterinary Medicine, 2002:42-44.
[23]PIEIRO M A, GYMNICH S B, KNURA S B, et al. Meat juice: an alternative matrix for assessing animal health by measuring acute phase proteins. Correlations of pig-MAP and haptoglobin concentrations in pig meat juice and plasma[J]. Research in Veterinary Science, 2009, 87(2):273-276.
[24]Witten I. Ways of evaluating the health status of slaughter pigs and its effect on the storage life of pork by using haptoglobin analysis[D]. Ph.D. thesis. Hannover: University of Veterinary Medicine, 2005:63-65.
[25]PETERSEN H H, DIDERIKSEN D, CHRISTIANSEN B M, et al. Serum haptoglobin concentration as a marker of clinical signs in finishing pigs[J]. Veterinary Record, 2002, 151(3): 85-89.
[26]ECKERSALL P D, DUTHIE S, TOUSSAINT M J, et al. Standardization of diagnostic assays for animal acute phase proteins[J]. Advances in Veterinary Medicine, 1999, 41:643-655.
京公网安备 11010802041926号