本文主要研究不同饱和程度油脂对瘤胃微生物体外产气及辅酶F420动态变化的影响。试验选用3头装有瘤胃瘘管的徐淮白山羊提供瘤胃液，分对照组、花生油组（A）、菜籽油组（B）、玉米油组（C）和豆油组（D）5个组进行体外培养，每组3个重复，每个重复1个培养瓶。结果表明：1) 36 h总产气量在20.61~39.67 mL间变化，除花生油组外，其他油脂组均显著低于对照组（P<0.05）；随时间的延长，各组产气量呈现波动变化，甲烷产量和甲烷/总产气量比率在组间差异显著（P<0.05），且甲烷/总产气量比率有从B到D逐渐下降的趋势。2) 对照组辅酶F420荧光值显著高于油脂组（P<0.05），除菜籽油组外，其他油脂组辅酶F420荧光值有随油脂不饱和程度增加而降低的趋势。3） 相关分析结果表明，辅酶F420荧光值与甲烷产量（0.916）、与总产气量（0.905）相关皆极显著（2-尾<0.01）。4） 豆油组总脱氢酶显著高于对照组，并依次显著高于玉米油、花生油、菜籽油组（P<0.05）。因此，随本试验所选油脂不饱和程度的加大，抑制甲烷产量和总产气量的效应也随之加大，并以豆油的效果较佳。
The objective of this paper was to assay the effect of different oils supplementation on dynamics of gas production and coenzyme F420 of ruminal microbe in vitro, using 3 Xuhuai white goats with permanent cannulas. Five groups were designed as follows: control group (no oil), peanut oil (A), rapeseed oil (B), corn oil (C), and soybean oil (D) respectively. Each group had 3 replicates and 1 fermentation bottle per replicate. The results showed as follows: 1) the volume of accumulated gas within 36 h was varied from 20.61 to 39.67 mL, except for the peanut oil group, the other oil groups were all significantly lower than the control group (P<0.05); the gas production of the 5 groups showed fluctuation with time prolonging and significant differences were found in methane production and methane to gas production ratio among groups (P<0.05), and methane to gas production ratio had a decreasing tendency from B to D. 2) Coenzyme F420 fluorescence value of the control group was higher than that of the oil groups (P<0.05), except for the rapeseed oil group, coenzyme F420 fluorescence value decreased with increasing degrees of oil unsaturation. 3) Good correlations were observed between coenzyme F420 fluorescence value and methane production (r=0.916), coenzyme F420 fluorescence value and gas production (r=0.905) (2-tail<0.01). 4) The total dehydrogenase activity of the soybean oil group was significantly higher than that of the control group, and was also higher than that of the corn oil, peanut oil and rapeseed oil groups in order (P<0.05). Finally, it could be concluded that, the inhibiting effects of the oil supplementation on coenzyme F420 fluorescence value, methane and gas production enhanced with increasing unsaturation degrees of the four kinds of oils, and soybean oil showed a remarkable effect comparatively.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1819 -1825］
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