芽孢乳杆菌对肉仔鸡生产性能、肠道发育和微生物菌群的影响

刘磊, 朱立贤

doi:10.3969/j.issn.1006-267x.2011.12.014

动物营养学报 >

2011 , Vol. 23 >Issue 12: 2136 - 2142

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.12.014

饲料营养

芽孢乳杆菌对肉仔鸡生产性能、肠道发育和微生物菌群的影响

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1. 山东农业大学动物科技学院,泰安 271018;
2. 山东农业大学食品科学与工程技术学院,泰安 271018

刘磊(1985—),男,山东青州人,博士研究生,从事家禽科学研究。E_mail: liusanshi1985@126.com

收稿日期: 2011-07-19

网络出版日期: 2011-12-14

基金资助

公益性行业(农业)科研专项经费项目(201003011)

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Effects of Sporolactobacillus on Performance, Intestinal Development and Microflora of Broilers

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1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. College of Food Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2011-07-19

Online published: 2011-12-14

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摘要

本试验旨在研究不同水平芽孢乳杆菌对肉仔鸡生产性能、肠道发育及微生物菌群的影响。选取1日龄爱拔益加(AA)肉仔鸡2 400只,随机分为4组(每组6个重复,每个重复100只):对照组饲喂基础饲粮,试验组饲喂分别添加200、100和50 mg/kg芽孢乳杆菌的试验饲粮,试验期42 d。结果表明:1)饲粮添加200 mg/kg的芽孢乳杆菌显著降低了肉仔鸡1~3周的料重比(P<0.05);添加100 mg/kg的芽孢乳杆菌显著降低了肉仔鸡全期的料重比和死淘率(P<0.05)。2)饲粮添加200 mg/kg的芽孢乳杆菌显著提高了21日龄肉仔鸡十二指肠和空肠的体重校正长度(P<0.05);添加100 mg/kg的芽孢乳杆菌显著提高了42日龄肉仔鸡十二指肠的相对重量(P<0.05)。3)饲粮添加100 mg/kg的芽孢乳杆菌显著增加了21日龄肉仔鸡空肠绒毛长度、隐窝深度、黏膜厚度和V/C值(P<0.05)。4)饲粮添加200、100和50 mg/kg的芽孢乳杆菌均能显著降低肉仔鸡空肠食糜中大肠杆菌的数量(P<0.05)。由此可见,饲粮添加200和100 mg/kg的芽孢乳杆菌能改善肉仔鸡的料重比和死淘率,效果优于50 mg/kg;添加100 mg/kg的芽孢乳杆菌能促进肠道发育,提高消化吸收功能;所有添加水平的芽孢乳杆菌均能抑制肉仔鸡空肠内的大肠杆菌繁殖。

关键词： 芽孢乳杆菌; 肉仔鸡; 生产性能; 肠道发育; 微生物区系

本文引用格式

刘磊, 朱立贤 . 芽孢乳杆菌对肉仔鸡生产性能、肠道发育和微生物菌群的影响[J]. 动物营养学报, 2011 , 23(12) : 2136 -2142 . DOI: 10.3969/j.issn.1006-267x.2011.12.014

Abstract

This experiment was conducted to investigate the effects of dietary Sporolactobacillus level on the performance, intestinal development and microflora of broilers. A total of 2 400 one-day-old broiler chickens (Arbor Acres) were randomly allocated to 4 groups with 6 replicates per group and 100 chickens per replicate. The broilers were randomly subjected to one of the following 4 treatments for 42 days: control group (fed a basal diet) and three treatments (fed the basal diet with 200, 100 and 50 mg/kg Sporolactobacillus, respectively). The results showed as follows: 1) adding 200 mg/kg Sporolactobacillus significantly decreased feed/gain of broilers at weeks 1 to 3 (P<0.05), and adding 100 mg/kg Sporolactobacillus significantly decreased feed/gain and mortality of broilers at weeks 1 to 6 (P<0.05). 2) Adding 200 mg/kg Sporolactobacillus significantly increased the weight correction length of duodenum and jejunum of broilers on day 21 (P<0.05). Adding 100 mg/kg Sporolactobacillus significantly increased the duodenal relative weight of broilers on day 42 (P<0.05). 3) Adding 100 mg/kg Sporolactobacillus significantly increased villus length, crypt depth, mucosal thickness and V/C value in jejunum of broilers on day 21 (P<0.05). 4) Adding 200, 100 and 50 mg/kg Sporolactobacillus all decreased significantly the number of E. coli in chyme of jejunum of broilers (P<0.05). In conclusion, adding both of 200 and 100 mg/kg Sporolactobacillus can decrease feed/gain and mortality of broilers, and the effects are better than those of adding 50 mg/kg Sporolactobacillus; adding 100 mg/kg Sporolactobacillus can improve intestinal development and the function of digestion and absorption; all supplemental levels of Sporolactobacillus can inhibit the reproduce of E. coli in jejunum of broilers.

Key words： Sporolactobacillus; broilers; performance; intestinal development; microflora

参考文献

[1] 王玉梅,李朝华,陆军,等.饲喂益生素对肉仔鸡生产性能及免疫水平的影响[J].饲料博览,2006(6):28-30.

[2] 马彦博.EM制剂在蛋鸡生产中的应用效果试验[J].家畜生态,2003,24(4):17-18.

[3] SALMINEN S, VON WRIGHT A, MORELLI L, et al. Demonstration of safety of probiotics—a review[J]. International Journal of Food Microbiology, 1998, 44(1/2):93-106.

[4] 潘康成,古丛伟,吴敏峰,等.饲用芽孢杆菌作用机理研究新进展[J].饲料与畜牧,2009(12):19-23.

[5] 王长文,娄玉杰,宋春梅.动物微生态营养理论的特性及其研究应用[J].吉林医药学院学报, 2008,29 (4):220-222.

[6] 杨海英,杨在宾,杨维仁,等.益生素和低聚木糖对断奶仔猪生产性能和肠道形态学影响研究[J].中国粮油学报,2008,23 (1):116-120.

[7] 吴建忠,杜冰,冯定远.乳酸芽孢杆菌制剂对AA肉鸡生产性能的影响[J].饲料工业,2007,28 (12):35-36.

[8] 杨革.微生物学实验教程[M].北京:科学出版社,2004:143-150.

[9] 段丽萍,陈洪,温燕,等.大鼠一过性肠道感染模型的建立[J].中国比较医学杂志,2003,13(3):142-145.

[10] 柯祥军,张小浔,瞿明仁,等.不同水平发酵豆粕对肉鸡肠黏膜结构的影响[J].饲料博览,2007(10):5-8.

[11] JIRAPHOCAKUL S, SULLIVAN T W, SHAHANI K M. Influence of a dried Sporolactobacillus subtilis culture and antibiotics on performance and intestinal microflora in turkeys[J]. Poultry Science, 1990, 69(11):1966-1973.

[12] 詹志春,胡水林,杨先奎,等.生物制品添加剂的研究应用:在肉用仔鸡前期和断奶仔猪日粮中的应用[J].粮食与饲料工业,1993(2):32-35.

[13] 何瑞国,张兆伟,王建兵,等.生长猪饲粮添加猪微生物添加剂与添加赖氨酸对比试验[J].四川农业大学学报,1994(S1):558-560.

[14] 刘磊,刘影,成廷水,等.不同菌株芽孢杆菌对肉鸡生产性能的影响[J].饲料工业,2010,31(12):49-51.

[15] 韩正康.家畜营养生理学[M].北京:农业出版社,1991:16-17.

[16] CASPARY W F. Physiology and pathophysiology of intestinal absorption[J]. The American Journal of Clinical Nutrition, 1992, 55(1):229-308.

[17] 郭元晟,闫素梅,史彬林,等.发酵乳酸杆菌对肉鸡小肠绒毛形态的影响[J].动物营养学报,2011,23(7):1194-1200.

[18] 王玉梅,李朝华,王雪莲.益生菌对肉仔鸡小肠组织结构和生产性能的影响[J].中国兽医科技,2006(11):59-60.

[19] ALEXOPOULOS C, KARAGIANNIDIS A, KRITAS S K, et al. Field evaluation of a bioregulator containing live Bacilli cereus spores on health status and performance of sows and their litters[J]. Journal of Veterinary Medicine Series A, 2001, 48(3):137-145.

[20] JADAMUS A, VAHIEN W, SIMON O. Growth behaviour of a spore forming probiotic strain in the gastrointestinal tract of broiler chicken and piglets[J]. Archives of Animal Nutrition, 2001, 54(1):1-17.

[21] GUO X, LI D, LU W, et al. Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs[J]. Antonie Van Leeuwenhoek, 2006, 90(2):139-146.

[22] KORNEGAY E T, RISLEY C R. Nutrient digestibilities of a corn-soybean meal diet as influenced by Bacillus products fed to finishing swine[J]. Journal of Animal Science, 1996, 74(4):799-805.

[23] HOSOI T, AMETANI A, KIUCHI K, et al. Improved growth and viability of Lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin[J]. Canadian Journal of Microbiology, 2000, 46(10):892-897.

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