为了开发瘤胃微生物酶资源，本研究以湖羊瘤胃为对象，构建瘤胃微生物的Fosmid文库，并对其库容量、稳定性和功能性进行分析。文库共获得克隆12 704个。通过Xho Ⅰ和BamH Ⅰ限制性酶切分析发现，文库插入片断平均为30.9 kb （17～55 kb），主要集中于36～40 kb和26～30 kb 2个区间；所建文库容量为393 Mb。Hind Ⅲ限制性分析证实，在连续传代100次时，文库仍保持很好的稳定性。通过刚果红染色分析获得18个具有木聚糖酶活性的阳性克隆。上述结果表明该文库具有较好的库容量、稳定性和功能性，可用于后续酶资源开发。

A Fosmid library of uncultured microorganism from Hu sheep rumen was constructed to explore the potential of the ruminants’ gut ecosystem， and the library storage capacity, stability and the functionality was also analyzed. A total of 12 704 clones were acquired from the Fosmid library. Restriction analysis by Xho Ⅰ and BamH Ⅰ revealed a high level of diversity of the cloned DNA fragments. The insert size of clone ranged from 17 to 55 kb, with average insert size of 30.9 kb, which mostly distributed between 36～40 kb and 26～30 kb. The capacity of this Fosmid library was 393 Mb. It was demonstrated by restriction analysis with Hind Ⅲ that the Fosmid library clones cultured for 100 generations still showed good stability. With the Congo red stain analysis, 18 clones had been acquired with the activity of xylanase. It was suggested that the constructed Fosmid library had good storage capacity, stability and the functionality, and could be used for further research on exploration of enzyme resource.［Chinese Journal of Animal Nutrition, 2010，22（2）:341-345］