本文旨在研究向日葵茎芯多糖（HAP）的提取工艺，向日葵茎芯原粉及HAP对鸡血清白介素-2（IL-2）含量的影响。采用索氏提取法从向日葵茎芯中提取多糖，选用1日龄爱拔益加（AA）肉仔鸡100只，按试验要求随机分为4组，每组5个重复，每个重复5只鸡。Ⅱ、Ⅲ、Ⅳ组饲喂基础饲粮，Ⅰ组在基础饲粮中添加600 mg/kg的向日葵茎芯原粉，Ⅲ组在肉仔鸡14日龄时颈部皮下连续7 d每天注射提取的多糖0.2 mL/只，Ⅳ组注射相同剂量的生理盐水作为Ⅲ组的对照。在21日龄时从每组鸡中随机抽取12只，翅静脉采血，分离血清，用鸡IL-2酶联免疫试剂盒检测血清IL-2含量。结果表明，向日葵茎芯多糖含量为5.341 6%；在基础饲粮中添加600 mg/kg的向日葵茎芯原粉可显著提高肉仔鸡血清中的IL-2含量（P<0.05）；对肉仔鸡颈部皮下每天注射0.2 mL/只的HAP连续7 d后，血清中的IL-2含量有所提高，但差异不显著（P＞0.05）。结果提示，饲粮中添加一定浓度的向日葵茎芯原粉可诱导机体产生IL-2。

An experiment was conducted to study the extraction process of Helianthus annuus polysaccharide (HAP) and the effects of sunflower stem core and HAP powder on chicken serum interleukin-2 (IL-2). Soxhlet’s extraction was adopted to extract HAP. One hundred one-day-old Arbor Acres broilers were randomly assigned to 4 groups, with 5 replicates per group and 5 broilers per replicate. Chickens in groups Ⅱ, Ⅲ and Ⅳ were fed with basal diet, while chickens in group Ⅰ were fed with the basal diet supplemented with 600 mg/kg sunflower stem core powder. Each bird in group Ⅲ was treated with subcutaneous injection of 2 mg HAP for 7 days, while each bird in group Ⅳ was injected with 2 mg normal saline as control. Measurements of IL-2 levels in serum were taken on 12 randomly selected birds of 21 days old in each group by Chicken IL-2 ELISA Kit. The results showed as follows: 1) the content of HAP was 5.341 6%; 2) the basal diet containing 600 mg/kg of sunflower stem core powder significantly increased the content of IL-2 in serum (P<0.05); 3) the content of serum IL-2 was increased 7 days post-injection of 2 mg HAP (P>0.05). These results indicate that the basal diet supplemented with sunflower stem core powder can induce the production of IL-2.［Chinese Journal of Animal Nutrition, 2010，22（5）:1440-1444］