本试验旨在研究益生菌与石灰复合处理玉米秸秆对东北细毛羊瘤胃发酵的影响。选用4只装有永久性瘤胃瘘管的成年雄性东北细毛羊,按4×4拉丁方试验设计,分别饲喂玉米秸秆(CK组)、6%石灰处理秸秆(A组)、益生菌处理秸秆(M组)、益生菌与石灰复合处理秸秆(C组),研究其对东北细毛羊瘤胃液pH、氨态氮(NH3-N)浓度、微生物蛋白质(MCP)产量和挥发性脂肪酸(VFA)浓度的影响。结果表明:各处理东北细毛羊瘤胃液pH均维持在正常范围内,组间差异不显著(P>0.05);C组瘤胃液NH3-N浓度、瘤胃MCP产量显著高于CK组(P<0.05);采食后2、4 h瘤胃总VFA浓度C组高于其他各组,但瘤胃平均总VFA浓度组间差异不显著(P>0.05)。益生菌与石灰复合处理玉米秸秆能更好地被反刍动物利用,有利于瘤胃发酵。
This experiment was conducted to study the effects of treating corn stalk with probiotics in combination with lime on rumen fermentation characteristics in Northeast fine-wool sheep. Four male sheep with permanent rumen fistula were used in a 4×4 Latin square design and fed with corn stalk (group CK), corn stalk treated with 6% lime (group A), corn stalk treated with probiotics (group M), and corn stalk treated with probiotics combined with lime (group C), respectively. Rumen pH, the concentrations of ammonia nitrogen (NH3-N), microbial crude protein (MCP) and volatile fatty acids (VFAs) were determined in this experiment. The results showed that the rumen pH of animals in the four groups were all in a normal range, and the differences among the four groups were not significant (P>0.05); the concentration of NH3-N and production of MCP in group C were significantly higher than those in group CK (P<0.05); the concentration of total volatile fatty acids (TVFAs) in group C was higher than that in the other groups at 2 and 4 h (P>0.05), but there were no significant differences in the average concentration of rumen TVFAs among the four groups (P>0.05). The results indicate that corn stalk treated by probiotics in combination with lime is more effective to improve rumen fermentation in sheep.[Chinese Journal of Animal Nutrition,2011,23(2):316-321]