本试验旨在研究饲料中不同水平的铜和维生素C交互作用对花鲈生长性能、肝脏铜积累量及免疫功能的影响。饲料设计适量（3.5 mg/kg）和高量（35 mg/kg）2个铜水平，3个维生素C添加水平（50、500和1 000 mg/kg），采用2×3交互设计，配制成6种等氮、等能的饲料用于各试验组，各组分别为S50、S500、S1000、H50、H500和H1000，每组3个重复，每个重复30尾鱼。试验周期为6周。结果表明：1）S500组花鲈的末重和特定生长率显著高于S1000组（P＜0.05）；S500、H50组肥满度显著高于S50组（P<0.05）。2)高铜组的肝脏铜积累量显著高于适铜组（P＜0.05）；交互作用下，高铜组显著高于S1000组(P<0.05)。3）高铜组肝脏中C3含量显著低于适铜组（P<0.10），交互作用下，H50组显著低于S50组（P<0.10）；S500组肝脏钠钾三磷酸腺苷酶活性最高，显著高于H1000组（P<0.10）；S500组肝脏碱性磷酸酶(AKP)活性最高，交互作用下，显著高于S50组（P<0.10）;适铜组的丙二醛（MDA）含量显著高于高铜组（P<0.10）。结果提示，花鲈饲料中添加3.5 mg/kg铜和500 mg/kg维生素C时，鱼体生长性能最好，肝脏的铜积累量较低，免疫功能得到提高。

This experiment was conducted to evaluate the effects of dietary copper-vitamin C interaction on growth performance, copper accumulation in the liver and immune function of Lateolabrax japonicus. Six isoenergetic and isonitrogenous semi-purified diets were prepared according to a 2×3 interaction design, which included 2 copper levels ［proper (3.5 mg/kg) and high (35 mg/kg)］, and 3 vitamin C levels (50, 500 and 1 000 mg/kg). The experimental groups were S50, S500, S1000, H50, H500 and H1000, which had 3 replicates per group and 30 fish per replicate. The experiment lasted for 6 weeks. The results showed as follows: 1) the final weight and specific growth rate (SGR) of group S500 were significantly higher than those of group S1000 (P<0.05); the condition factors (CF) of groups S500 and H50 were significantly higher than that of groups S50 (P<0.05). 2) The copper accumulations in the liver of high copper level groups were significantly higher than those of proper level groups (P<0.05), and were significantly higher than those of group S1000 under interaction (P<0.05). 3) The complement 3 (C3) content in the liver of high copper level groups was significantly lower than that of proper copper level groups （P<0.10）, and that of group H50 was significantly lower than that of group S50 under interaction （P<0.10）; the liver Na+-K+-ATPase activity of group S500 was the highest, and was significantly higher than that of group H1000 （P<0.10）; the liver alkaline phosphatase (AKP) activity of group S500 was the highest, and was significantly higher than that of group S50 under interaction （P<0.10）; the malonaldehyde （MDA） content of proper copper level groups was significantly higher than that of high copper level groups （P<0.10）. The results indicate that fish with the supplementation of copper at 3.5 mg/kg and vitamin C at 500 mg/kg in diet have the best growth performance and the lower copper accumulation in the liver, the immune function is also improved.［Chinese Journal of Animal Nutrition，2011，23（3）：395-402］