本文旨在探讨谷氨酰胺（Gln）对断奶仔猪抗菌肽PR-39 mRNA表达的影响。选取64头胎次相同、28日龄断奶、体重 (7.50±0.67) kg的三元杂交仔猪（杜×长×大），随机分为2个组，即基础日粮对照组和1.0% Gln添加组，每组设4个重复，每个重复8头猪（公母各1/2）。于试验第28天，进行细胞因子白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)和肿瘤坏死因子(TNF-α)含量的分析。于第30天，采用半定量RT-PCR研究日粮中添加Gln对PR-39 mRNA的影响。试验结果表明：Gln添加显著提高仔猪血清中IL-1β含量（P<0.05），但对血清IL-6和TNF-α及空肠黏膜TNF-α、IL-2无显著影响（P>0.05）；显著促使骨髓、空肠黏膜中PR-39 mRNA的表达（P<0.05），与对照组相比，骨髓和空肠黏膜中PR-39 mRNA分别提高50.5%（P<0.05）和24.0%（P<0.05）。由结果可知，断奶仔猪日粮中添加Gln能显著促使骨髓及空肠黏膜PR-39 mRNA表达上调。

To investigate the effects of glutamine（Gln） on expression of antibacterial peptide PR-39 mRNA in weaned piglets. Sixty four 28-day-old weaned piglets （Duroc×Landrace×Yorkshire） with an initial body weight of (7.50±0.67) kg were randomly allocated to two groups (control group and 1.0% Glnadded group) with four replicates in each group of eight per replicate (half male and half female). On day 28, the levels of IL-1β, IL-6 and TNF-α were analysed. On day 30, the effects of Gln on expression of antibacterial peptide PR-39 mRNA were determined by semiquantitative RT-PCR. The results indicated that, the level of IL-1β in serum increased significantly (P<0.05) with Gln addition, however, the levels of IL-6, TNF-α in serum and TNF-α, IL-2 in mucosal of jejunum were not significant affected with Gln addition compared with control group (P>0.05); the relative expressions of PR-39 mRNA in femur marrow and mucosal of jejunum significantly increased by 50.5% (P<0.05) and 24.0% (P<0.05), respectively. It was concluded, addition of Gln to diets for weaned piglets could significantly increase relative expression of PR-39 mRNA in both femur marrow and mucosal of jejunum.