本文简要介绍了尤斯灌流（Ussing chamber）系统在动物胃肠道屏障及营养物质转运中的应用，同时提出了在使用Ussing chamber过程中发现的一些问题。建议在应用Ussing chamber系统过程中应使离体研究环境尽量符合胃肠道生理特征，使研究结果更为准确并且更具生理意义，同时通过研究方法的改进使研究材料成本降低。

This article introduced the basal components and structures of Ussing chamber and its applications in the field of gastrointestinal barrier function and nutrient transport research. During the process of applying Ussing chamber system, some problems had been found and the solutions had been proposed. It was suggested that in vitro environment needed to be accorded with gastrointestinal physiological characteristics for accurate experimental results and meaningful physiological simulation, simultaneously, material cost was decreased by improvement of methods.［Chinese Journal of Animal Nutrition, 2010，22（3）:511-518］