为研究不同饲喂方式对猪背最长肌钙蛋白酶抑制蛋白（calpastatin）和μ-钙蛋白酶（μ-calpain）mRNA丰度，以及对肌肉剪切力的影响，54头约60 kg的杜×长×大三元杂交去势公猪被随机分为3个组，每组3个重复，每个重复6头猪。第1组为对照组，自由采食；第2组为补偿生长组，前20 d限饲30%，后20 d自由采食；第3组为限饲组，全期限饲30%。所有猪饲养40 d后屠宰取样，用实时定量PCR测定猪背最长肌中calpastatin和μ-calpain mRNA相对丰度。结果表明，背最长肌calpastatin和μ-calpain mRNA相对丰度在自由采食组和补偿生长组间差异不显著（P>0.05）；在补偿生长组和限饲组间差异显著（P<0.05）；在自由采食组和限饲组间差异极显著（P<0.01）。背最长肌剪切力与calpastatin表达量显著正相关，相关系数为0.505 9。背最长肌剪切力与μ-calpain相对丰度显著负相关，相关系数为-0.475 7。背最长肌中calpastatin mRNA相对丰度与μ-calpain mRNA相对丰度负相关，相关系数为-0.055 9。结果表明补偿生长和限饲均可以增强calpastatin和μ-calpain基因在转录水平的调控。

To investigate the effects of different feeding methods on gene expression of calpastatin and μ-calpain and shear force in longissimus dorsi muscle of pigs, fifty-four Duroc×Landrace×Large White tribasic hybrid barrows with average weight of about 60 kg were randomly allocated into 3 groups with 3 replicates each and 6 pigs per replicate. The first group was taken as control group with ad libitum feeding, and the second group was taken as compensatory growth group in which feed was restricted by 30% for initial 20 days and then was ad libitum feeding for final 20 days, and the third group was restricted feeding group in which feed was restricted by 30% during whole experimental period. All pigs were slaughtered and samples were collected, and relative abundance of calpastatin and in μ-calpain mRNA in longissimus dorsi muscle of pigs were determined by real time PCR. The results showed as follows: the relative abundance of calpastatin and μ-calpain mRNA in longissimus dorsi muscle had no significant difference between ad libitum feeding group and the compensatory growth group (P>0.05), and there was significant difference between the compensatory growth group and the restricted feeding group (P<0.05), and between ad libitum feeding group and the restricted feeding group (P<0.01). The shear force of longissimus dorsi muscle was positively correlated with calpastatin mRNA significantly (correlation coefficient=0.505 9). There was negative correlation between shear force of longissimus dorsi muscle and relative abundance of μ-calpain mRNA (correlation coefficient=-0.475 7) and between relative abundance of calpastatin mRNA and μ-calpain mRNA (correlation coefficient=-0.055 9). It was concluded that compensatory growth and restricted feeding both strengthened regulation of calpastatin and μ-calpain gene at transcriptional level.［Chinese Journal of Animal Nutrition, 2010，22（3）:640-646］