本研究旨在通过营养调控、添加益生素或牛至油，探讨替代抗生素，提高猪群抗病、抗应激能力的高效绿色添加剂组合。试验选用平均体重27 kg，健康长×大杂交阉公猪324头，随机分为6组：Ⅰ组（金霉素组，在基础日粮中添加50 mg/kg金霉素）、Ⅱ组（牛至油组，在基础日粮中添加20 mg/kg牛至油）、Ⅲ组（益生素组，在基础日粮中添加1.5 g/kg益生素）、Ⅳ组（营养调控组，提高日粮中维生素E和B族维生素、微量元素Zn、Mn和I水平）、Ⅴ组（牛至油+营养调控组）、Ⅵ组（益生素+营养调控组）。试验期为90 d。结果表明：Ⅴ组猪生长速度最快，料重比最低，平均日增重显著高于Ⅳ组和Ⅵ组（P<0.05）；血液中白细胞数量Ⅲ组最高，显著高于Ⅳ组和Ⅴ组（P<0.05）；淋巴细胞转化率Ⅲ组和Ⅴ组较高，显著高于Ⅰ组、Ⅱ组和Ⅳ组（P<0.05）；Ⅰ组谷胱甘肽过氧化物酶（GSH-Px）活性最高，Ⅴ组过氧化氢酶（CAT）活性最高；猪瘟、蓝耳病及口蹄疫抗体水平组间差异不显著（P>0.05）。本研究结果表明，营养调控同时添加牛至油完全可以替代抗生素，猪的生长性能、抗应激和抗病力超过添加金霉素。

This experiment was conducted to explore the feasibility of substituting for antibiotics with probiotics, origanum oil and through elevating dietary vitamin E, vitamin B family and micro elements levels. Three hundred and twenty-four crossbred (Landrace×Yorkshine) barrows with average body weight of 27 kg were randomly assigned to 6 dietary groups: group Ⅰ (chlorotetracycline group, added 50 mg/kg chlortetracycline in basal diet); group Ⅱ (origanum oil group, added 20 mg/kg oreganum oil in basal diet); group Ⅲ (probiotics group, added 1.5 g/kg probiotics in basal diet); group Ⅳ (nutrient enhancement group, elevated dietary vitamin E, vitamin B family and micro elements Zn, Mn, I); group Ⅴ (origanum oil+nutrient enhancement group); group Ⅵ (probiotics+nutrient enhancement group). The trial lasted for 90 days. The results showed that pigs in group Ⅴ recorded the highest ADG, which was significantly higher than that in group Ⅳ and group Ⅵ (P<0.05), and had the best F/G. The number of white blood cells in group Ⅲ was significantly higher than that in group Ⅳ and group Ⅴ (P<0.05). The lymphocyte transformation rate in group Ⅲ and group Ⅴ was significantly higher than that in group Ⅰ, group Ⅱ and group Ⅳ (P<0.05). The activity of GSH-Px in group Ⅰ was the highest, while the activity of CAT in group Ⅴ was the highest. Antibody levels of classical swine fever, porcine reproductive and respiratory syndrome and foot-and-mouth disease had no difference among groups (P>0.05). These results indicated that origanum oil+nutrient enhancement could successfully substitute for antibiotics, and pigs fed diets added origanum oil and elevated dietary vitamin E, vitamin B family and micro elements could obtain better performance, have better disease-resistant and stress-resistant ability than pigs fed diets supplemented with chlortetracycline.［Chinese Journal of Animal Nutrition, ２００9，２1（5）:645-651］