本试验选用1日龄艾维茵肉仔鸡448只,随机分为8组,每组4个重复,每个重复14只鸡,采用4×2(Cu×VA)完全随机设计,研究了日粮添加不同水平的铜(0、8、150、225 mg/kg)和维生素A(1 500、5 000 IU/kg)对肉仔鸡不同生长阶段(前期0~4周龄和后期5~7周龄)的生长性能及血液生理指标的影响。结果表明:高铜(150、 225 mg/kg)显著降低前期体增重和采食量、血红蛋白生成和血沉(P<0.05);高铜有利于白细胞生成 (P<0.05);0 mg/kg铜组显著提高血红蛋白含量、后期红细胞压积和前期血沉(P<0.05);铜的不同添加水平对全期红细胞数目影响不显著(P>0.05)。5 000 IU/kg维生素A组获得较好的生长性能、血红蛋白含量、红细胞数目,并显著提高前期红细胞压积和白细胞数目,但显著降低后期红细胞压积(P<0.05)。铜和维生素A互作效应对前期体增重及全期料重比影响均显著(P<0.05),对前后期血红蛋白含量、血沉、白细胞计数及后期红细胞压积影响均极显著(P<0.01),且二者间存在互补作用,Cu(8 mg/kg)×VA(5 000 IU/kg)组与Cu(0 mg/kg)×VA(5 000 IU/kg)组对前期肉仔鸡生长性能、红细胞计数、血红蛋白含量、红细胞压积、血沉均较好。铜和维生素A互作效应对全期红细胞数目影响不显著(P>0.05)。结果提示,在基础日粮铜水平为16~23 mg/kg时,铜的适宜添加量前期为8 mg/kg,后期为0~8 mg/kg;全期维生素A的添加量为5 000 IU/kg。
A 4×2 (Cu×VA) factorial arrangement with replicates was designed to investigate the effects of different dietary copper (0, 8, 150, 225 mg/kg diet) and vitamin A levels (1 500, 5 000 mg/kg diet) and their interaction on growth performance, hemoglobin (Hb) concentration, red cell count (RCC), packed cell volume (PCV), erythrocyte sedimentation rate (ESR) and white cell count (WCC) in broilers. Broilers were randomly allotted to eight groups with four replicated in each group and fourteen broilers in each replicate. Results showed dietary higher copper levels (150 and 225 mg/kg) decreased significantly body weight gain, feed intake, Hb concentration and ESR from 0 to 4 weeks, and improved WCC significantly in the whole period (P<0.05). When dietary copper supplemental level was 0 mg/kg, Hb concentration in the whole period, PCV from 5 to 7 weeks and ESR from 0 to 4 weeks improved significantly (P<0.05), but different dietary copper levels did not significantly affect RCC in the whole period (P>0.05). When vitamin A was 5 000 IU/kg, better growth performance, Hb concentration and RCC in the whole period were gained, and PCV and WCC from 0 to 4 weeks increased significantly (P<0.05), but 5 000 IU/kg vitamin A significantly decreased PCV from 5 to 7 weeks (P<0.05). Cu VA interaction significantly affected body weight gain from 0 to 4 weeks and feed/gain in the whole period (P<0.05), and significantly affected Hb concentration, ESR and WCC in the whole period (P<0.01), PCV from 5 to 7 weeks (P<0.01), and they had complementary effect, both Cu (8 mg/kg)×VA (5 000 IU/kg) and Cu (0 mg/kg)×VA (5 000 IU/kg) had better growth performance, RCC, Hb concentration, PCV and ESR from 0 to 4 weeks. Cu VA interaction didn’t significantly affected RCC in the whole period (P>0.05). When copper level in basal diets was 16~23 mg/kg, dietary appropriate copper supplemental level was 8 mg/kg from 0 to 4 weeks, and 0~8 mg/kg from 5 to 7 weeks. Appropriate vitamin A supplemental level was 5 000 IU/kg in the whole period.