This study was to explore the establishment of model of iron deficiency in weanling piglet and to observe the change of growth performance, blood biochemical indices and the iron content of the organ after supplementation iron-enriched yeast or ferrous sulfate. Thirty-five 21-day-old weanling D×L×Y piglets with an average weight of (5.57±0.83) kg, were randomly allocated into 7 groups with 5 replicates (1 pig per replicate), and piglet was fed in single cage. Piglets were fed the basal diet included 22.43 mg/kg iron for 4 weeks to consume reserving iron in body. After iron was exhausted, piglets in control group were fed the basal diet continuously, and piglets in 6 trial groups were fed the basal diet supplemented with 80, 120 and 180 mg/kg iron either from iron-enriched yeast or ferrous sulfate during trial period. The trial duration was 10 days after 28 days depletion period. The results showed that piglets’ model of iron deficiency was successfully established after 28 days. When iron concentration was 120 mg/kg, ADG and ADFI of piglets were the highest in all groups. There were significant effects on interactions of iron sources and levels on ADG and F/G (P<0.05). There were no significant effects on interactions of iron sources and levels on blood routine and blood biochemical indices (P>0.05). Both iron-enriched yeast or ferrous sulfate of 120 mg/kg iron concentration significantly increased iron content in the internal organs, including spleen, liver, kidney and heart compared with control group (P<0.01); while iron concentration in ferrous sulfate with 120 mg/kg iron group significantly higher than that in iron-enriched yeast group (P<0.01). ADG, ADFI, serum ferritin and transferrin in iron-enriched yeast group were higher than those in ferrous sulfate group. In conclusion, both iron-enriched yeast and ferrous sulfate could improve the state of iron deficiency, and effect of iron-enriched yeast was better than that of ferrous sulfate.［Chinese Journal of Animal Nutrition, 2009，21(6):897-902］