An eight-week feeding trial was conducted to evaluate the effects of different sources of trace elements on growth, metabolism and non-specific immunity of tilapia (Oreochromis niloticus). The basal diet was formulated to contain crude protein 32% and gross energy 14 MJ/kg, as control diet (diet 1). Three experimental diets were formulated containing iron, zinc, manganese and copper from the inorganic trace elements (diet 2), amino acid chelated trace elements (diet 3) and complex amino acid chelated trace elements (diet 4) with the same basal diet, respectively. Diets 3 and 4 contained the same amount of trace elements (iron, zinc, manganese and copper) at a half level in diet 2, respectively. Each diet was fed to triplicate tanks of genetically improved farmed tilapia [(average body weight of (27.2±0.1) g], and each tank was stocked with 30 fish. The results showed as follows: trace elements (iron, zinc, manganese and copper) supplementation could significantly improve the growth performance, activities of metabolic enzymes in hepatopancreas and non-specific immunity of tilapia (P<0.05). Fish fed the diet containing amino acid chelated trace elements or complex amino acid chelated trace elements had significantly higher specific growth rate, protein efficiency ratio, activities of glutamic pyruvic transaminase, glutamic oxalacetic transaminase, total superoxide dismutase and CuZn-superoxide dismutase in hepatopancreas, and activities of catalase, alkaline phosphatase and lysozyme in serum than fish fed the diet containing inorganic trace elements (P<0.05). The results suggest that the amino acid chelated trace elements can improve the growth performance and non-specific immunity of tilapia and they are more bioavailable than the inorganic trace elements. Therefore, amino acid chelated trace elements can become a safe and efficacious additive supplemented to the diets for tilapia.［Chinese Journal of Animal Nutrition, 2011, 23（5）：763 -770］
LIN Shimei，PAN Yu，LUO Li，MA Xia，ZHAN Ruorui，MAO Shuhong
. Effects of Different Sources of Iron, Zinc, Manganese and Copper on Growth, Metabolism and Non-specific Immunity of Tilapia (Oreochromis niloticus)[J]. Chinese Journal of Animal Nutrition, 2011
, 23(05)
: 763
-770
.
DOI: 10.3969/j.issn.1006-267x.2011.05.009
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