This study was conducted to isolate the lactic acid bacteria and investigate its characteristics of lactate production in the rumen of goat. Six strains of lactic acid-producing bacteria (named L2, L3, L5, L8, L10 and L12) were isolated from the rumen contents of goats with the methods of anaerobic isolation technics, morphology and 16S RNA sequence analysis. L2, L8 and L10 were identified as Streptococcus bovi., L5 was identified as Streptococcus equinus, and L3 and L12 were identified as Weissella cibaria. L3 and L12 were the first time isolated from the rumen to obtain. The results of growth curves showed that the strain entered a stationary phase of growth after 10 h incubation. After 24 h incubation, the concentrations of L-lactate of L3, L5, L8, L10 and L12 were all beyond 14 mmol/L; the concentrations of D-lactate of L3, L5 and L12 were all beyond 14 mmol/L; the concentrations of D-lactate of L2, L10 and L8 were 4.17, 5.07 and 0.83 mmol/L, respectively. The results indicate that the Streptococcus bovis, Streptococcus equinus and Weissella cibaria are the predominant lactate producer in the rumen of goats, and they can produce both the D- and L-lactate.［Chinese Journal of Animal Nutrition, 2011, 23（6）：965 -970］
WEI Deyong,ZHU Weiyun,MAO Shengyong
. Isolation and Identification of Lactic Acid Bacteria and Characteristics of D- and L-lactate Production in Goat Rumen[J]. Chinese Journal of Animal Nutrition, 2011
, 23(06)
: 965
-970
.
DOI: 10.3969/j.issn.1006-267x.2011.06.012
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