Abstract: This study was conducted to investigate the percentage of the total digestibility of amylase, trypsin, chymotrypsin and lipase to that of all enzymes in jejunal fluid in Beijing ducks in vitro, as well as the influence of the other enzymes changing on the test results. A single-factor completely randomized design was used. Three treatments with same activities of the four main digestive enzymes were set according to the digestive enzymes composition of intestinal fluid. Treatment 1 was endogenous intestinal fluid of ducks (4 main digestive enzymes＋the other digestive enzymes), treatment 2 was a mixture of intestinal fluid dry powder and reagent enzymes (4 main digestive enzymes＋the other digestive enzymes), and treatment 3 was reagent enzymes (4 main digestive enzymes). Each treatment contained 5 replicates and 1 simulative digestive tube per replicate. The dry matter and energy digestibility of 4 feed ingredients (corn, soybean meal, cottonseed meal and wheat bran) and 4 diets were determined. The results showed as follows: 1) there were significant effects of different treatments on the dry matter and energy digestibility of feed ingredients and diets (P<0.05), but the effects on different experimental material were not coincident; 2) the dry matter and energy digestibility in treatment 2 ranged from 100.09% to 101.76% and 100.82% to 103.52% of those in treatment 1, respectively; 3) the dry matter and energy digestibility in treatment 3 ranged from 89.38% to 110.85% and 86.39% to 115.63% of those in treatment 1, respectively. The results indicate that the percentage of the total digestibility of the four main digestive enzymes to that of all the digestive enzymes in duck intestinal fluid was over 86.39%, and the interference of the other enzymes’ changing was 1.72%; the preparation of simulative intestinal fluid is an ideal method that using the endogenous intestinal fluid purification powder as the main ingredient, and adjusting the activities of the four main enzymes to the same levels as endogenous intestinal fluid with regent enzymes.［Chinese Journal of Animal Nutrition, 2011, 23（6）：991 -997］
XIE Mulin,ZHAO Feng,ZHANG Hongfu,ZHANG Shiyuan
. A Comparative Study on in vitro Digestibility of All and Main Digestive Enzymes in the Duck Jejunal Fluid[J]. Chinese Journal of Animal Nutrition, 2011
, 23(06)
: 991
-997
.
DOI: 10.3969/j.issn.1006-267x.2011.06.016
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