The mammary nutrition research in lactating ruminants, especially in dairy cows, has become a hot spot of animal nutrition research at present, and mammary blood flow is one of the key factors that affect the absorption, uptake and utilization of mammary nutrients, therefore, the accurate measurement of mammary blood flow has the theoretical and practical significance. This article took the mammary gland of dairy goats as the object and the transit-time ultrasonic blood flow meter was used to assess the measurement of mammary blood flow and the accuracy under different conditions. The results showed that external pudendal vein ligation could effectively prevent external pudendal vein blood refluxed into the mammary vein and did not affect the mammary blood flow (P>0.05); the effect of nutrition on mammary blood flow was slow but significant (P<0.01); milking had little effect on mammary blood flow (P>0.05), but lying could increase mammary blood flow significantly (P<0.01); increasing the feeding times could reduce mammary blood flow fluctuations and enhance the rate of accuracy of measuring mammary blood flow, the mammary blood flow was decreased gradually from 01:00 to 12:00 o’clock and then increased, at the same time the milk yield was in a positive correlation with the average of the blood flow. In conclusion, the external pudendal vein ligation can effectively prevent the blood flow measurement from inaccuracy; nutrition has a great effect on the mammary blood flow; milking has little effect on the blood flow while lying can increase blood flow significantly; the measurement of mammary blood flow will be more accurate under the conditions of increasing the feeding times.[Chinese Journal of Animal Nutrition, 2011, 23(6):1035 -1042]
ONG Yifu,MA Weiming*,WANG Zhonghua,HE Jiashuang,FU Ying,CAO Yongzhi,CHI Hongliang,XIE Bing,LIU Lianlian
. Measurement of Mammary Blood Flow in Dairy Goats[J]. Chinese Journal of Animal Nutrition, 2011
, 23(06)
: 1035
-1042
.
DOI: 10.3969/j.issn.1006-267x.2011.06.022
[1]GOREWITA R C, AROMANDOA M C, BRISTOL D G. Measuring bovine mammary gland blood flow using a transit-time ultrasonic flow probe[J]. Journal of Dairy Science, 1989, 72(7):1981-1928.
[2]CHRISTENSEN K, NIELSEN M O, BAUER R, et al. Evaluation of mammary blood flow measurements in lactating goats using the ultrasound doppler principle[J]. Comparative Biochemistry and Physiology Part A: Physiology, 1989, 92(3):385-392.
[3]DHONT G, HOUVENAGHEL A, PEETERS G, et al. Influence of vasoactive hormones on blood flow through the mammary artery in lactating cows[J]. Archives Internationales de Pharmacodynamie et de Therapie, 1973, 201(1):195-196.
[4]DROST C J. Vessel diameter-independent volume flow measurements using ultrasound[J]. Proceedings San Diego Biomedical Symposium, 1978, 17:299-302.
[5]LINZELL J L. The measurement of udder blood flow in the conscious goat[J].Journal of Physiology, 1957, 137:75-76.
[6]RASMUSSEN F. The mammary blood flow in the cow as measured by the antipyrine absorption method[J]. Acta Veterinaria Scandinavica, 1965, 6:135-149.
[7]REYNOLDS M, LINZELL J L, RASMUSSEN F. Comparison of four methods for measuring mammary blood flow in conscious goats[J]. American Journal of Physiology, 1968, 214(6):1415-1424.
[8]DELAMAIRE E, GUINARD-FLAMENT J. Increasing milking intervals decreases the mammary blood flow and mammary uptake of nutrients in dairy cows[J]. Journal of Dairy Science, 2006, 89:3439-3446.
[9]PEARL S L, DOWNEY H F, LEPPER T L. Intramammary pressure and mammary blood flow in lactating goats[J]. Journal of Dairy Science, 1973, 56:1319-1323.
[10]LOUGH D S, BEEDE D L, WILCOX C J. Effects of feed intake and thermal stress on mammary blood flow and other physiological measurements in lactating dairy cows[J]. Journal of Dairy Science, 1990, 73:325-332.
[11]NIELSEN M O, NYBORG S, JAKOBSEN K, et al. Mammary uptake and excretion of prostanoids in relation to mammary blood flow and milk yield during pregnancy-lactation and somatotropin treatment in dairy goats[J]. Domestic Animal Endocrinology, 2004, 27:345-362.
[12]RENAUDEAU D, LEBRETON Y, NOBLET J, et al. Measurement of blood flow through the mammary gland in lactating sows: methodological aspects[J]. Journal of Animal Science, 2002, 80:196-201.
[13]LACASSE P, FARR V C, DAVIS S R, et al. Local secretion of nitric oxide and the control of mammary blood flow[J]. Journal of Dairy Science, 1996, 79:1369-1374.
[14]THIVIERGE M C, PETITCLERC D, BERNIER J F, et al. External pudic venous reflux into the mammary vein in lactating dairy cows[J]. Journal of Dairy Science, 2000, 83:2230-2238.
[15]王俊锋,李兵,李海燕,等.真胃梯度酪蛋白钠灌注对奶山羊泌乳性能和乳腺组织氨基酸吸收利用的影响[J].动物营养学报,2007,19(4):333-343
[16]NIELSEN M O, JAKOBSEN K, ANDERSON P H. Validity of mammary blood flow measurements relying on manual clamping of the pudendal vein[J]. Livestock Production Science, 1993, 35:179-180.
[17]BEQUETTE B J, BACKWELL F R C, CROMPTON L A. Current concepts of amino acid and protein metabolism in the Mammary Gland of the Lactating Ruminant[J]. Journal of Dairy Science, 1998, 81:2540-2559.
[18]LACASSE P, PROSSER C G. Mammary blood flow does not limit milk yield in lactating goats[J]. Journal of Dairy Science, 2003, 86:2094-2097.
[19]DAVIS S R, COLLIER R J. Mammary blood flow and regulation of substrate supply for milk synthesis[J] Journal of Dairy Science, 1985, 68:1041-1058
[20]RENAUDEAU D, LEBRETON Y, NOBLET J, et al. Measurement of blood flow through the mammary gland in lactating sows: methodological aspects[J]. Journal of Animal Science, 2002, 80:196-201.
[21]HOUVENAGHEL A, PETERS G, VERSCHONTEN F. Influences of manual udder stimulation and oxytocin on mammary artery blood flow in lactating cows[J]. Archives Internationales de Pharmacodynamie et de Therapie, 1973, 205:124-133.
[22]RAJCZYK Z K, SWEETING A, LEAN I J, et al. Postural effects on mammary blood flow and nutrient uptake[J]. Proceedings of the Nutrition Society of Australia, 1995, 19:119.