This experiment was conducted to study the influence of soybean agglutinin (SBA) on the structure and function of carp intestinal epithelial cells in primary culture. The carp intestinal epithelial cells in primary culture were cultured in 24-well plate after 72 h, then changed into the DMEM culture medium which contained 0, 50, 75, 100, 150 and 200 μg/mL purified SBA, respectively. The samples were collected to measure the indices after 72 h SBA culture. The results showed that the activities of lactate dehydrogenase (LDH) and glutamic-pyruvic transaminase (GPT) in culture medium of carp intestinal epithelial cells were significantly enhanced by SBA (P<0.01), but the influence on glutamic-oxalacetic transaminase (GOT) activity in the culture medium didn't reach significant level (P>0.05). The results of cell morphological observation showed that the cell shape was obviously changed by SBA, and it was changed from ellipse to long strip or irregular shape and the number of dead cells in culture medium increased. Meanwhile, the SBA could significantly increase the methyl thiazolyl tetrazolium optical density (MTT OD) value and protein content (P<0.05 or P<0.01) in carp intestinal epithelial cells, and significantly enhance the activities of GOP and GPT in cells and ammonia concentration in culture medium（P<0.05 or P<0.01）, but significantly decrease the activities of alkaline phosphatase (AKP) and Na+，K+-ATPase in cells (P<0.01). These results indicate that SBA can damage the normal structure of cells, promote the proliferation and apoptosis of cells, restrain cell differentiation and accelerate the protein metabolism for carp intestinal epithelial cells.［Chinese Journal of Animal Nutrition, 2011, 23（7）：1140 -1146］
FENG Lin,JIANG Jun,LIU Yang1HU Kai,JIANG Weidan,ZHOU Xiaoqiu
. The Influence of Soybean Agglutinin on the Structure and Function of Carp Intestinal Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2011
, 23(07)
: 1140
-1146
.
DOI: 10.3969/j.issn.1006-267x.2011.07.011
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