This research was conducted to investigate the optimal supplemental level of chromium picolinate for heat-stressed Jian carp (Cyprinus carpio var. Jian). A total of 360 Jian carp with average initial weight of (32.88±0.17) g were randomly divided into 6 groups with 4 replicates per group and 15 fish per replicate. Fish in the 6 groups were fed with the basal diet supplemented with different levels of chromium picolinate, and the theoretical supplementation levels of Cr3+ in the 6 experimental diets were 0, 300, 600, 900, 1 200 and 1 500 μg/kg, respectively. The experiment was carried out in indoor facilities with water recycling system which has a temperature control unit. The acclimatization period lasted for 10 d (26 ℃) and the trial period lasted for 60 d (‘normal temperature-high temperature-normal temperature’ stress model: 20 d, 26 ℃; 20 d, 31 ℃; 20 d, 26 ℃). The results showed as follows: 1) supplementation of 600 and 900 μg/kg chromium picolinate (calculated as Cr3+, the same as below) in diet could significantly increase weight gain and protein efficiency, and decrease feed conversion for Jian carp (P＜0.05). Compared with the control group, weight gain rate of fish fed diets supplemented with 1 500 μg/kg chromium picolinate decreased by 3.4% (P＞0.05). With the increase of chromium picolinate supplementation, the hepatosomatic index of Jian carp was increased, and had a significantly (P＜0.05) higher when fish fed the diets supplemented with 900, 1 200 and 1 500 μg/kg chromium picolinate. 2) The contents of ether extract, ash and moisture in muscle and hepatopancreas ether extract were not significantly affected by chromium picolinate supplementation (P＞0.05), while the crude protein content in muscle and hepatopancreas in 300 and 600 μg/kg supplemental groups was significantly higher than that in control group (P＜0.05). 3) Supplementation of 600 μg/kg chromium picolinate could significantly increase the serum albumin content (P＜0.05), while 1 500 μg/kg chromium picolinate could significantly decrease the contents of serum albumin and total protein (P＜0.05), 600, 900, 1 200 and 1 500 μg/kg chromium picolinate could significantly decrease the serum cholesterol content (P＜0.05), and 900, 1 200 and 1 500 μg/kg chromium picolinate could significantly decrease the serum triacyglycerol content (P＜0.05). It is concluded that the supplementation of 600 μg/kg chromium picolinate (measured value of Cr3+ is 912.94 μg/kg diet) has a better anti-heat stress effect, which can improve growth and increase muscle protein content of heat stressed Jian carp. ［Chinese Journal of Animal Nutrition, 2011, 23（7）：1209 -1216］
WANG Jingjing,LIU Wenbin,XU Weina,WANG Ying,ZHANG Weiwei
. The Optimal Supplemental Level of Chromium Picolinate for Heat Stressed Jian Carp (Cyprinus carpio var. Jian)[J]. Chinese Journal of Animal Nutrition, 2011
, 23(07)
: 1209
-1216
.
DOI: 10.3969/j.issn.1006-267x.2011.07.020
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