Ruminant Nutrition

Effects of Different Plant Oils on the Enzyme Activity and Microbial Activity in vitro

Expand
  • 1. College of Animal Science and Technology, Yangzhou University, Yangzhou  225009, China; 2. Gannan Normal College, Ganzhou 341000, China

Online published: 2011-08-15

Abstract

The objectives of this paper were to determine the effects of different plant oils on the enzyme activity and microbial activity in vitro. Three goats with permanent cannulas were used in a simple factor design. The control group was supplemented with no oil and the experimental groups were supplemented with peanut oil, rapeseed oil, corn oil, and soybean oil, respectively. The results showed that the activity of lactate dehydrogenase (LDH), glutamic oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) were highest in rapeseed oil group and that in soybean oil group, corn oil group and peanut oil group fell in turn, the activity of LDH, GOT and GPT in the experimental groups were significant higher than that in the control group except for peanut oil group (P<0.05) and there were significantly differences among the experimental groups (P<0.05). The activity of total dehydrogenase in soybean oil group was the highest and significantly higher than that of the control group (P<0.05) and that in corn oil group, peanut oil group and rapeseed oil group fell in turn. Additionally, no significant difference was observed in the protozoal DNA, bacterial DNA, microbial DNA, and protozoa to bacteria ratio among groups (P>0.05). However, microbial DNA was generally higher in soybean oil group and corn oil group at all sampling time points, and the changing trend along with time differed from each other. It was further observed that, protozoa to bacteria ratios were much lower in soybean and corn oil. In conclusion, different oils had different effects on microflora and activity of ruminal microorganisms in vitro, while soybean oil and corn oil showed much better effects on promoting microbial activity than the others. [Chinese Journal of Animal Nutrition, 2011, 23(8):1309 -1316]

Cite this article

WANG Shu,WANG Mengzhi,LU Zhanjun,DONG Shuhong,ZHANG Xin,WANG Hongrong . Effects of Different Plant Oils on the Enzyme Activity and Microbial Activity in vitro[J]. Chinese Journal of Animal Nutrition, 2011 , 23(08) : 1309 -1316 . DOI: 10.3969/j.issn.1006-267x.2011.08.009

References

[1]DIJKSTRA J, GERRITS W J J, BANNINK A, et al. Modelling lipid metabolism in the rumen[C]//Modelling Nutrient Utilization in Farm Animals, MCNAMARA J P, FRANCE J, BEEVER D E, eds. Publisher: CABI Publishing, 2000.

[2]MCGINN S M, BEAUCHEMIN K A, COATES T. Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid[J]. Journal of Animal Science, 2004, 82:3346-3356.

[3]王梦芝,程欣,谢文文,等.体外法研究不同油脂对瘤胃原虫吞噬细菌微循环的影响[J].中国农业科学,2010,43(8):3831-3837.

[4]BUSQUET M, CALSAMIGLIA S, FERRET A, et al. Effect of garlic oil and four of its compounds on rumen microbial fermentation[J]. Journal of Dairy Science, 2005, 88: 4393-4404

[5]李旦,王加启,卜登攀,等.运用Real-time PCR方法研究日粮添加豆油与胡麻油对肉牛瘤胃纤维分解菌数量的影响[J].动物营养学报,2008,20(3):256-260.

[6]JALCˇD, CˇERTIK M, KUNDRIKOVA K, et al. Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen[J]. Czech Journal of Animal Science, 2009, 54(5): 229-237.

[7]MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research and Development, 1988, 28:7-55.

[8]HUMEYAN D B, NAGARAJA T G, MILLER G W, et al. Rumen microbial changes in cattle fed diets with or without salinomycin[J]. Applied and Environmental Microbiology, 1986, 51(2):340-345.

[9]赵亚华.生物化学与分子生物学实验技术教程[M].北京:高等教育出版社,2005.

[10]JENKINS T C. Lipid metabolism in the rumen[J]. Journal of Dairy Science, 1993, 76:3851-3863.

[11]JALCˇ D, KIIDAYOV S, NERUD F. Effect of plant oils and organic acids on rumen fermentation in vitro[J]. Folia Microbiologic, 2002, 47(2):171-177.

[12]加勒特,格里萨姆.生物化学[M].3版.北京:高等教育出版社,2005:809-853.

[13]OWENS F N, SECRIST D S, HILL W J, et al. Acidosis in cattle: a review[J]. Journal of Animal Science, 1998, 76:275-286.

[14]ENEMARK J M, JRGENSEN R J, ENEMARK P. Rumen acidosis with special emphasis on diagnostic aspects of subclinical rumen acidosis: a review[J]. Veterinary Zootechnique, 2002, 20(42):16-29.

[15]胡红莲,刘大程,卢德勋,等.日粮不同NFC/NDF比对奶山羊血液参数的影响[J].江西农业大学学报,2008,30(5):855-859.

[16]刘春龙,李杰.丝兰皂甙对绵羊瘤胃原虫数目及酶活性的影响[J].西南农业大学学报,2005,27(2):214-217.

[17]程茂基,卢德勋,王洪荣,等.不同来源肽对培养液中瘤胃细菌蛋白产量的影响[J].畜牧兽医学报,2004,35(1):1-5.

[18]李莉.应用微生物学[M].武汉:武汉理工大学出版社,2006.

[19]NGUYEN T H N, NGUYEN T N, NGUYEN T, et al. Determination of the optimum level of a soybean oil drench with respect to the rumen ecosystem, feed intake and digestibility in cattle[J/OL]. Livestock Research for Rural Development, 2007, 19(8). [2011-02-01]. http://www.cipav.org.co/lrrd/lrrd19/8/nhan19117.htm.

[20]WANG M Z, WANG H R, YU L H. Effects of NDF content on protozoal community and grazing rate in rumen[J]. Journal of Animal and Veterinary Advances, 2009, 8(9): 1746-1752.

[21]陈蛋,陈斌,陆道礼,等.近红外光谱分析法测定菜籽油中芥酸的含量[J].农业工程学报,2007,23(1):234-237.
 
Outlines

/