This experiment was conducted to study the requirements of vitamins and amino acids for Apis cerana cerana during overwintering period. Based on the contents of vitamins and amino acids in honey, we designed the experimental diets, including 67.28 mg/kg vitamin complex and 127.85 mg/kg amino acid complex group (group A1B1), 67.28 mg/kg vitamin complex and 256.70 mg/kg amino acid complex group (group A1B2), 134.56 mg/kg vitamin complex and 127.85 mg/kg amino acid complex group (group A2B1), 134.56 mg/kg vitamin complex and 256.70 mg/kg amino acid complex group (group A2B2). The effects of dietary levels of vitamin complex and amino acid complex were studied, honey and sugar (syrup) served as control, and the feed consumption, reduction rate of population of swarm and antioxidant activity were detected. The results showed as follows: 1) the reduction rate of population of group A1B2 and the control group D (honey) was significantly lower than that of the control group C (syrup) (P<0.05), and there was not significantly different between group A1B2 and the control group D (P>0.05); the reduction rate of population of group A2B1 was significantly higher than that of the control group D (P<0.05), but there was no significant difference compared with the other groups (P>0.05). There was no effect of different levels of vitamin complex and amino acid complex on feed consumption compared with the control group C (syrup) and D (honey) (P>0.05). 2) The total superoxide dismutase (T-SOD) activity of group A1B1 was significantly higher than that of group A1B2, A2B2 and the control group C and D (P<0.05), while there was no significantly different in T-SOD activity between group A1B1 and group A2B1 (P>0.05). There was no effect of different levels of vitamin complex and amino acid complex on malondialdehyde (MDA) content (P>0.05). In conclusion, by adding the vitamin complex and amino acid complex in syrup, Apis cerana cerana can obtain adequate nutrition during overwintering period, and the supplemental levels of vitamin complex should be lower than 67.28 mg/kg and that of amino acid complex should be from 127.85 to 256.70 mg/kg.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1756 -1761］
LIU Junfeng,WU Xiaobo,HE Xujiang,TIAN Liuqing,ZENG Zhijiang
. Requirements of Vitamins and Amino Acids for Apis cerana cerana during Overwintering Period[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1756
-1761
.
DOI: 10.3969/j.issn.1006-267x.2011.10.015
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