The study was conducted to investigate the effect of dietary chitosan nanopartides loaded copper (CNP-Cu) on growth performance, immunity and antioxidant indices of weaned piglets. A total of 108 21-day-old three way cross (Duroc×Landrace×Yorkshine) weaned piglets with an initial average body weight of (7.20±0.81) kg were randomly assigned into 6 groups with 3 replicates per group and 6 piglets per replicate. The piglets in control group were fed basal diet; Piglets in chlortetracycline (CTC) group were fed the basal diet supplemented with 100 mg/kg CTC, and piglets in four trial groups （Ⅰ, Ⅱ, Ⅲ and Ⅳ） were fed the basal diet supplemented with 50, 100, 150 and 200 mg/kg CNP-Cu, respectively. The experiment lasted for 28 days. After termination of the feeding trial, blood samples were collected from anterior vena cava of all piglets for serum indices analysis. The results showed as follows: 1) supplementation of CNP-Cu increased average daily feed intake and average daily gain, and decreased feed gain ratio and diarrhea rate, and the optimal supplemental level of CNP-Cu was 100 mg/kg (P<0.05). 2) Serum complement component 3, glutathione peroxidase activity and total antioxidant capacity were significantly increased with the supplementation of CNP-Cu (P<0.05). These results suggest that supplemental CNP-Cu has beneficial effects on immune and antioxidant function in weaned pigs, which also can increase growth performance and decrease diarrhea rate. Accordingly, CNP-Cu has a potential to substitute antibiotics.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1806 -1811］
WANG Minqi,YE Shanshan,DU Yongjie,TAO Wenjing,XIE Xiaoli
. Chitosan Nanoparticles Loaded Copper Ions Affect Growth Performance, Immunity and Antioxidant Indices of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1806
-1811
.
DOI: 10.3969/j.issn.1006-267x.2011.10.022
［1］王润莲，贾志海，朱晓萍.甲壳素和壳聚糖营养研究进展[J].动物营养学报，2006，18(4):299-302.
[2]DU W L, NIU S S, XU Y L, et al. Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions[J]. Carbohydrate Polymers, 2009, 75:385-389.
[3]杜文理.载金属离子氨基多糖纳米微粒的抗菌作用及其对大鼠免疫功能的影响[D].博士学位论文.杭州：浙江大学，2008.
[4]BRAUDE R. Some observations on the need for copper in the diet of fattening pigs[J]. The Journal of Agricultural Science, 1945, 35:163-167.
[5]CROMWELL G L, STAHLY T S, MONEGUE H J. Effects of source and level of copper on performance and liver copper stores in weanling pigs[J]. Journal of Animal Science, 1989, 67(11):2996-3002.
[6]COFFEY R D, CROMWELL G L, MONEGUE H J. Efficacy of a copper-lysine complex as a growth promotant for weanling pigs[J]. Journal of Animal Science, 1994, 72(11):2880-2886.
[7]许梓荣，吴新民.不同化学形式的铜对仔猪生长、消化和胴体组成的影响[J].动物营养学报，1999，11(3):29-35.
[8]王荣梅，苏荣胜，曹华斌，等.不同形式和剂量的铜对肉鸡生长速度和料肉比的影响[J].饲料研究，2009，9:5-6，11.
[9]QI L F, XU Z R, JIANG X, et al. Preparation and antibacterial activity of chitosan nanoparticles[J]. Carbohydrate Research, 2004, 339:2693-2700.
[10]刘梅，史挺.壳聚糖对肉仔鸡生长性能及血清生化指标的影响[J].粮食与饲料工业，2009，10:41- 42.
[11]ZHANG C F, SHI B L, JIN X, et al. Effects of dietary supplementation of chitosan on growth performance and serum IGF-Ⅰand GH in piglets[J]. Chinese Journal of Animal Nutrition, 2008, 20(2):191-195.
[12]史彬林，李德发，朴香淑.壳聚糖对肉仔鸡生长性能及免疫功能的影响[J].中国畜牧杂志，2005，41(1)：9-11.
[13]TANG Z R, YIN Y L, NYACHOTI C M, et al. Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-Ⅰ mRNA expression in early-weaned piglets[J]. Domestic Animal Endocrinology, 2005, 28:430-441.
[14]陈旭东，胥传来，马秋刚，等.金霉素、果寡糖和芽孢杆菌对断奶仔猪生产性能和血清学指标的影响[J].中国畜牧杂志，2005，41(6)：25-27.
[15]耿春银，孙健，孙铁镇，等.纳豆菌微生态制剂替代抗生素用于断奶仔猪饲料的研究[J].中国饲料，2010，15：24-27.
[16]DU W L, XU Y L, XU Z R, et al. Preparation, characterization and antibacterial properties against E.coli K88 of chitosan nanoparticle loaded copper ions[J]. Nanotechnology, 2008, 19(8). doi: 10.1088/0957-4484/19/8/085707.
[17]HUSSAIN N. Fluorometric method for the simultaneous quantitation of differently-sized nanoparticles in rodent tissue[J]. International Journal of Pharmaceutics, 2001, 214(1/2):55-61.
[18]WANG Y Z, XU C L, AN Z H, et al. Effect of dietary bovine lactoferrin on performance and antioxidant status of piglets[J]. Animal Feed Science and Technology, 2008, 140(3/4):326-336.
